益气方干预化疗所致免疫功能低下作用机理的研究  

The Mechanism of Yiqi Formula on Chemotherapy Induced Immune Dysfunction

作　　者：苏颖[1] 郭焱[1] 朱娜[1] 顾红缨[1] 李春子[1] 

机构地区：[1]长春中医药大学,吉林长春130117

出　　处：《中国中医基础医学杂志》2010年第12期1116-1118,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：吉林省教育厅科研资助项目(200805132)

摘　　要：目的:分析益气方对环磷酰胺(CY)化疗所致小鼠白细胞水平、T淋巴细胞体外增殖和粒细胞集落刺激因子活性的影响,探讨其干预化疗所致免疫功能低下作用的机理。方法:小鼠给予CY致免疫功能低下,采用自动血液分析仪检测化疗小鼠白细胞水平;采用淋巴细胞转化实验(MTT法)检测小鼠T淋巴细胞的增殖程度;采用ELISA试剂盒测定粒细胞集落刺激因子活性,观察益气方对化疗小鼠免疫功能低下的治疗作用。结果:给予CY后,模型组各项指标均低于空白对照组,证明造模成功。益气方高、中、低剂量组与模型组比较各指标均有显著性差异(P<0.05或P<0.01),其中中剂量组疗效最好。结论:益气方对化疗所致免疫功能低下有上调作用。Objective：To investigate the role of Yi-Qi Formula on white blood cells,T lymphocyte proliferation and granulocyte colony-stimulating factor activity of cyclophosphamide（CY） chemotherapy in mice,and to explore the mechanism of intervention effect of chemotherapy-induced immune function.Methods： Mice were given CY induced immunosuppression,white blood cells in mice were analyzed by using automated hematology analyzer;using lymphocyte transformation test（MTT） assay to analyze T lymphocytes proliferation in mice;measured granulocyte colony stimulating factor activity by ELISA kit,to observe Yi-Qi Formula n treatment on immune function of chemotherapy in mice.Results： After giving CY,model group were lower than the control group,proved successful model.Yi-Qi Formula of high,medium and low dose group compared with the model group were significantly different（P 0.05 or P 0.01）,which is best effect in the dose group.Conclusion： The results suggest that Yi-Qi Formula has increased effect on immunosuppression induced by chemotherapy.

关 键 词：益气方 免疫功能低下 作用机理 

分 类 号：R285.5[医药卫生—中药学]