COPD大鼠气道黏液分泌调控机制及“从肠论治”干预作用  

Effect of "Treating Intestine" Method on Signal Transduction Pathway of Airway Mucus Secretion in Rats with Chronic Obstructive Pulmonary Disease

作　　者：钟相根[1] 李宇航[1] 张前[1] 贾旭[1] 孙燕[1] 郑丰杰[1] 谢华[1] 刘晓辉[1] 王蔚[1] 祝小惠[1] 周晓卫[1] 田彦[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中国中医基础医学杂志》2010年第12期1123-1125,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家重点基础研究发展规划(973计划)项目(2009CB522704);教育部新世纪优秀人才支持计划(NCET-08-0745)

摘　　要：目的:探讨"从肠论治"抑制慢性阻塞性肺疾病(COPD)模型大鼠气道黏液高分泌的调控机制,从黏液高分泌角度探讨COPD"从肠论治"的效应机制。方法:采用气管注射脂多糖加熏香烟联合造模方法建立COPD大鼠模型,随机分为正常组、模型组、治肺组、治肠组及肺肠同治组。正常组、模型组灌胃生理盐水,各给药组灌胃相应中药,连续14d。RT-PCR法检测支气管组织表皮生长因子受体(EGF-R)mRNA及白介素-13(IL-13)mRNA表达水平。结果:与正常组比较,模型组大鼠气道EGF-R mRNA、IL-13 mRNA表达增加(P<0.01);与模型组比较,治肠组EGF-R mRNA、IL-13 mRNA表达减少(P<0.05);与治肺组比较,肺肠同治组EGF-R mRNA、IL-13 mRNA表达减少(P<0.01)。结论:通利大肠或在治肺的基础上增加通利大肠,均能降低EGF-R mRNA、IL-13 mRNA表达,从而抑制气道黏液高分泌信号传导通路,这可能是COPD"从肠论治"效应产生的作用环节之一。Objective：To observe the influence ＂treating from intestine＂ on the airway mucus secretion signal transduction pathway in rats with COPD,and to investigate the effect of ＂treating from intestine＂from airway mucus hypersecretion.Methods： The rat model of COPD was established by using the method of cigarette smoking combined with intratracheal injection of LPS.All rats were randomly divided into normal control group,model group,treating lung group,treating intestine group,treating lung and intestine group.The normal control group,model group were given intragastrically the normal saline solution,while other groups were given corresponding herbal drugs intragastrically for 14 days.Detected the expressions of EGF-R、IL-13 mRNA in lung by RT-PCR.Results： Compared with normal control group,the expressions of EGF-R、IL-13 mRNA in airways of model group rats were markedly higher（P 0.01）.Compared with model group,the expressions of EGF-R、IL-13 mRNA of intestine group were lower（P 0.05）.Compared with lung group,the expressions of EGF-R、IL-13 mRNA of lung-intestine group were lower（P 0.01）.Conclusion： ＂catharsis large intestine＂can reduce the expression of EGF-R、IL-13 mRNA,therefore inhibits the airway mucus hypersecretion.

关 键 词：慢性阻塞性肺疾病 黏液分泌 宣白承气汤 从肠论治 白介素-13 表皮生长因子受体 

分 类 号：R562[医药卫生—呼吸系统]