超声造影定量分析组织血流灌注参数评价肿瘤化疗疗效  

Quantitative analyzing tissue perfusion parameters with contrast enhanced ultrasound in the evaluation of tumor chemotherapy

作　　者：周建华[1] 郑玮[1] 操隆辉[2] 韩峰[1] 刘敏[1] 李安华[1] 

机构地区：[1]华南肿瘤学国家重点实验室暨中山大学肿瘤医院超声科,广州510060 [2]华南肿瘤学国家重点实验室暨中山大学肿瘤医院麻醉科,广州510060

出　　处：《中华医学超声杂志（电子版）》2010年第9期3-6,共4页Chinese Journal of Medical Ultrasound(Electronic Edition)

摘　　要：目的探讨超声造影定量分析组织血流灌注参数在评价肿瘤化疗疗效中的作用。方法 18只小鼠建立H22肝癌皮下移植瘤模型,随机分为治疗组和对照组,每组各9只。自模型建立次日起分别经腹腔注射顺铂1mg/kg体重(顺铂治疗组)和0.5%羧甲基纤维素钠(CMC)(对照组),每天1次,连续给药5d。超声造影检查前,常规二维超声测量肿瘤3个最大径线,然后计算肿瘤体积。肿瘤接种后第8天行超声造影检查,然后脱机采用时间-强度曲线软件进行分析,获取曲线下面积(AUC)、峰值强度(IMAX)、灌注指数(PI)、平均通过时间(mTT)、峰值时间(TTP)和拟合优度(GOF)等参数。超声检查完成后完整剥离肿瘤,免疫组化检测肿瘤CD34表达情况,计算肿瘤微血管密度。结果与对照组相比,顺铂治疗组的肿瘤体积明显缩小(P=0.000)。顺铂治疗组的PI、IMAX和MVD均明显低于对照组(P<0.05),但AUC、mTT和TTP在两组之间的差异无统计学意义。结论超声造影定量分析组织血流灌注参数的改变可以有效评价肿瘤化疗的疗效。Objective To evaluate the value for quantitative analyzing tissue perfusion parameters of contrast-enhanced ultrasound in the evaluation of tumor chemotherapy.Methods Kun-min mouse were subcutaneously implanted with H22 cells.Nine mice were treated with cisplatin（1 mg /kg once daily） by intraperitoneal injection over 5 days,starting at day 2 after-implantation.Nine mice as control were treated with an equivalent volume of 0.5% CMC.Tumor＇s three largest dimensions were measured by conventional B mode ultrasound and then tumor volume was calculated according to volume formula.Contrast-enhanced gray-scale ultrasound was performed on day 8 after bolus injection of SonoVue（0.1 ml） and the imaging was recorded on cine.Regions of interest within tumour were analyzed off-line with the software of SonoLiver to determine the area under the curve（AUC）,maximum intensity（IMAX）,perfusion index（PI）,mean transit time（mTT）,time to peak（TTP） and quality of fit（QOF）.Mice were euthanized immediately after imaging and tumour tissue was removed for fixation in a 10% formalin solution.Microvascular density（MVD） was measured after anti-CD34 staining.Results The tumor volume of treated groups were significant different（lower） than that of control group（P = 0.000）.Treatment with cisplatin led to a significant decrease in PI and IMAX compared with control group（P 0.05）.There were no significant differences in AUC,mTT,TTP and QOF between control and treated tumours（P 0.05）.Treated tumours were associated with a significantly lower MVD compared with control tumours（P = 0.000）.Conclusion Quantitative analyzing tissue perfusion parameters of contrast enhanced ultrasound was indicated for monitoring tumor response to chemotherapy.

关 键 词：超声检查 造影剂 肿瘤 药物疗法 血流灌注 

分 类 号：R445.1[医药卫生—影像医学与核医学]