光动力疗法对体外培养人胰腺癌细胞株PANC-1作用  

Effect of photodynamic therapy on human pancreatic cancer cell line PANC-1 cultured in vitro

作　　者：徐晓军[1] 张振玉[1] 

机构地区：[1]南京医科大学附属南京第一医院消化科,江苏南京210006

出　　处：《现代生物医学进展》2010年第24期4658-4660,共3页Progress in Modern Biomedicine

基　　金：南京市科技局基金发展项目(2006ZD0117)

摘　　要：目的:探讨血卟啉注射液(Hematoporphyrin Derivative,HPD)光动力疗法(Photodynamic Therapy,PDT)对体外培养的人胰腺癌细胞株PANC-1的生物作用。方法:实验分为4组,空白对照组、单纯HPD组、单纯光照组及HPD+PDT组。采用MTT法检测光动力作用后细胞的存活率,并用Annexin V-FITC/P I双染法检测其凋亡率。结果:在光敏剂浓度为5mg/L,光照剂量为10J/cm2时,光动力对PANC-1细胞达到最佳的实验效果,与对照组相比差异有显著性。在此实验参数条件下,流式细胞术(FCM)检测各组人胰腺癌细胞PANC-1凋亡率:HPD+PDT实验组达(36.40±4.21)%,明显高于单纯HPD(6.76±0.44)%,单纯PDT组(8.30±0.32)%及空白组(5.00±0.53)%三个对照组(P<0.05),三个对照组间差异无统计学意义(P>0.05)。结论:PDT光动力作用对体外培养人胰腺癌细胞PANC-1有明确抑制效应,并与HPD浓度及光照强度相关。Objective： To investigate the effect of HPD injection（Hematoporphyrin Derivative,HPD） Photodynamic Therapy（Photodynamic Therapy,PDT） on cultured human pancreatic cancer cell line PANC-1.Methods： There were 4 groups： control group,simply HPD group,the simple light group and HPD ＋ PDT group.The cell survival was detected by MTT assay after photodynamic,and the apoptosis was detected by double staining with Annexin V-FITC/PI.Results： It achieved the best experimental results of the PANC-1 cells in the photosensitizer concentration of 5mg / L,light dose of 10J / cm2,the optical power,which was significant different with that of the control group.The PANC-1 human pancreatic cancer cell apoptosis was detected by the flow cytometry（FCM） ： HPD ＋ PDT of the experimental group was（36.40 ± 4.21）%,which was significantly higher than those of HPD（6.76 ± 0.44）%,PDT alone group（8.30 ± 0.32）% and control groups（5.00 ± 0.53）% in control group（P 0.05）,while the three control groups no significant difference（P 0.05）.Conclusion： Photodynamic therapy has clear inhibitory effect on cultured human pancreatic cancer cells PANC-1 and is related with the HPD concentration and light intensity.

关 键 词：光动力疗法 胰腺癌细胞 凋亡 

分 类 号：R454.2[医药卫生—治疗学] R735.9[医药卫生—临床医学]