载阿霉素PLGA纳米微球的制备及性质研究  

Study on Preparation of Adriamycin-loaded PLGA Nanoparticles in Vitro

作　　者：刘杰[1] 徐伟华[1] 金成[1] 张福琴[1] 窦科峰[1] 

机构地区：[1]第四军医大学西京医院肝胆胰脾外科,陕西西安710032

出　　处：《现代生物医学进展》2010年第24期4661-4663,共3页Progress in Modern Biomedicine

摘　　要：目的:制备新型癌症化疗制剂载阿霉素(Adriamycin)、聚乳酸-羟基乙酸共聚物(PLGA)纳米微球(ADM-PLGA-NP),研究其性质及体外释药特点。方法:以聚乳酸-羟基乙酸共聚物为包封材料,阿霉素为模型药物,采用复乳蒸发法制备ADM-PLGA-NP,扫描电镜观察微球形态,激光粒度分析仪检测粒径分布,紫外分光光度法计算载药率及包封率,体外药物释放实验考察微球对ADM的缓释作用。结果:ADM-PLGA-NP外观呈球形,平均粒径约(237±12.7)nm,载药量及包封率分别为(6.42±1.67)%和(53.82±8.34)%,药物在体外缓慢释放,5 d累积释放量达85%。结论:通过复乳蒸发法制备的ADM-PLGA-NP性质稳定,具有药物缓释性,有望成为一种新型的药物化疗载体。Objective： To prepare adriamycin-loaded poly（D,L-lactide-co-glycolide） nanoparticles（ADM-PLGA-NP） and to inves-tigate the characteristics and drug-release properties of ADM-PLGA-NPs in vitro.Methods： ADM-PLGA-NPs were prepared by w/o/w emulsification-solvent evaporation method,in which PLGA was as embedding materials,adriamycin was as embedded drug.The mor-phology and diameters were detected by scanning electron microscopy（SEM） and dynamic light scatter（DLS）,respectively.The encap-sulating efficiency of ADM was determined by ultraviolet spectrophotometry.Nanoparticles were put in a dialysis bag to observe the releasing properties of ADM in vitro.Results： ADM-PLGA-NPs were spherical shape with average diameter of（237 ± 12.7） nm.The entrapment efficiency and loading efficiency were（53.82±8.34） % and（6.42±1.67） %,respectively.85% of ADM encapsulated in ADM-PLGA-NPs was sustained released for 5 days.Conclusions： ADM-PLGA-NP displayed common stable characteristics and sus-tained drug release properties,and it can be regarded as an effective chemotherapy drug delivery system.

关 键 词：阿霉素 乳酸-羟基乙酸共聚物 纳米微球 缓释 

分 类 号：R965[医药卫生—药理学]