CSPGs在大鼠脊髓损伤后的表达及其与GFAP的关系  

The expression of chondroitin sulphate proteoglycans and its relationship with astrocyte after rat spinal cord compression injury

作　　者：安俊[1] 段丽[2] 张欲凯[2] 张海峰[2] 刘新秦[1] 沈学锋[1] 

机构地区：[1]第四军医大学预防医学院军队劳动与环境卫生学教研室 [2]第四军医大学神经科学研究所,陕西西安710032

出　　处：《现代生物医学进展》2010年第24期4669-4671,共3页Progress in Modern Biomedicine

基　　金：陕西省社发攻关项目(2006K152G1)

摘　　要：目的:观察脊髓损伤后CSPGs的表达及其与GFAP的关系。方法:成年雄性SD大鼠25只,随机分为对照组和损伤组,损伤组分脊髓挤压损伤后0h、72h、1w、4w组,运用免疫荧光双重染色方法观察CSPGs与GFAP的表达。结果:挤压伤后损伤部位的CSPGs和GFAP的表达均增高,但二者的变化趋势并不一样。其中CSPGs从损伤后表达开始增高,此后一直增加,并在1w至4w时逐渐稳定,主要分布逐渐集中于损伤部位;星形胶质细胞的免疫反应也逐渐增加,其分布逐渐集中于损伤区域的边缘,逐渐形成胶质瘢痕界膜。损伤1w至4w,损伤区域内几乎没有了星形胶质细胞表达,但仍留有大量的CSPGs。结论:早期抑制星形胶质细胞分泌CSPGs,可以防止在损伤部位沉积大量的CSPGs,从而减小其对再生纤维的抑制作用。Objective： To investigate the time course and distribution of chondroitin sulphate proteoglycans（CSPGs） and glial fib-rillary acidic protein（GFAP）.Methods：A total of 25 Sprague-Dawley rats were divided into control and injured group.At 0h,72h,1w,4w after compression injuries of the spinal cord,the expression of CSPGs and GFAP were detected by double-stained immunofluores-cence analysis.Results： The expression of both CSPGs and GFAP within the injury area of the spinal cord were increased,but the time course of expression of the two molecules was different.The expression of the CSPGs was increased immediately,and all along,after the injury,culminated at 1w,and spatially concentrated at the site of primary injury gradually.The immunoreaction of the GFAP was in-creased after injury,too,but concentrated at the margin of the injury site at last.One week after the compression injury of the spinal cord,there was little astrocyte at the site of primary injury,but were plenty of CSPGs accumulated.Conclusion： It may prevent the accumula-tion of CSGs in the injury area after spinal cord compression to inhibit the CSGs secreting from adtrocyte early after injury,which further attenuate the inhibitory effect of CSGs on the regeneration of axon after spinal cord injury.

关 键 词：脊髓损伤 硫酸软骨素蛋白多糖 星形胶质细胞 胶质瘢痕 

分 类 号：R95-3[医药卫生—药学] R651.2