北方汉族人群白细胞介素-6基因-572C/G单核苷酸多态性与冠心病的相关性研究  

Association of Interleukin-6 Gene-572C/G Single Nucleotide Polymorphism with Coronary Artery Disease in Han Population of North China

作　　者：梁振洋[1,2] 张效林[2] 孙莹[3] 冯雪瑶[4] 白净[1] 闫承慧[2] 康建[2] 韩雅玲[2] 

机构地区：[1]第四军医大学西京医院心内科,陕西西安710061 [2]沈阳军区总医院心内科,辽宁沈阳110016 [3]辽宁医学院,辽宁锦州121001 [4]大连医科大学,辽宁大连116044

出　　处：《现代生物医学进展》2010年第24期4675-4678,共4页Progress in Modern Biomedicine

基　　金：全军医学科学技术研究"十一五"计划课题项目(06G021)

摘　　要：目的:探讨中国北方汉族人群中白细胞介素-6(IL-6)基因启动子区-572C/G单核苷酸多态性与冠心病的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测434例冠心病(冠心病组)患者和417名非冠心病人(对照组)的IL-6基因型,探讨-572C/G单核苷酸多态与冠心病的关系。结果:IL-6基因-572C/G多态位点的CC、CG和GG基因型在冠心病组中分别为59.68%、37.09%、3.23%,等位基因频率C和G分别为78.23%、21.77%;在健康成年者中基因型分别为67.87%、30.22%、1.92%,等位基因频率C和G分别为82.97%、17.03%。IL-6基因-572C/G位点多态性在两组人群中的分布差异存在显著性(P<0.05);经Logsitic回归校正性别、年龄、体重指数、高血压病、糖尿病、高胆固醇血症及吸烟等冠心病易患因素后,IL-6基因-572C/G单核苷酸多态是冠心病发病的独立的危险因素(P<0.05);等位基因频率的相对风险分析发现,G等位基因携带者患冠心病的风险是C等位基因的1.356倍〔OR=1.356,95%CI=1.0648～1.7279〕。结论:在中国北方汉族人群中IL-6基因-572C/G单核苷酸多态性与冠心病的发病具有相关性,其等位基因G是冠心病重要的遗传学标记。Objective： To investigate the relationship between the Interleukin-6（IL-6） gene promoter region-572C / G single nu-cleotide polymorphism and coronary artery disease in Han population of North China.Methods： Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method was used to detect the IL-6 genotype in 434 cases of coronary artery disease（CAD） patients and 417 non-coronary artery disease（control group） and the relationship between-572C/G single nucleotide polymorphism and coronary heart disease was discussed.Results： IL-6 gene-572C / G polymorphism of the CC,CG and GG genotypes in the CAD group was 59.68%,37.09%,3.23%,while C and G allele frequencies were 78.23%,21.77%,respectively.Genotype in healthy adults who were 67.87%,30.22%,1.92%,C and G allele frequencies were 82.97%,17.03%.The distribution of IL-6 gene-572C/G polymorphism in the two groups was significant difference（p0.05）;After adjustment of sex,age,body mass index,hypertension,diabetes,high cholesterol,smoking and other conventional risk factors with Logsitic regression analysis,IL-6 gene-572C / G single nucleotide polymorphism was an independent coronary artery disease risk factors（P 0.05）;With the relative risk of allele frequency analysis,the risk of cornoary artery disease was markedly higher in the G allel carriers than that in the C allele carriers（OR=1.356,95%CI=1.0648～1.7279）.Conclusion： IL-6 gene-572C/G single nucleotide polymorphism is correlated with the incidence of cornoary artery disease in Han population of North China and G allele is an important genetic markers with coronary heart disease.

关 键 词：冠状动脉疾病 白细胞介素-6 启动子 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病]