曲美他嗪人血浆蛋白结合率的测定  

Determination of Binding Rate of Trimetazidine and Human Plasma Protein

作　　者：吕江[1] 祁英杰[1] 毕开顺[1] 果德安[2] 陈晓辉[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]中国科学院上海药物研究所,上海201203

出　　处：《现代生物医学进展》2010年第24期4751-4754,共4页Progress in Modern Biomedicine

摘　　要：目的:建立测定曲美他嗪与人血浆蛋白结合率的高效液相色谱法(HPLC),测定曲美他嗪在人血浆中的蛋白结合率。方法:采用平衡透析法,结合HPLC测定曲美他嗪在人血浆中的蛋白结合率。结果:低、中、高3个浓度下,曲美他嗪的血浆蛋白结合率分别为14.7%,16.2%和15.5%。结论:本方法灵敏度高,重现性好,操作简单,能满足生物样品分析的要求。曲美他嗪与血浆蛋白结合率较低。Objective： To develop an HPLC method to determine the trimetazidine in human plasma and to analysise the plasma protein binding rate of trimetazidine.Methods： The equilibrium dialysis combined with HPLC was carried out to determine of the plasma concentration and plasma protein binding rate of trimetazidine.Result： Human plasma protein binding rates were 14.7 %,16.2 % and 15.5 % when the trimetazidines were low,middle and high,respectively.Conclution： The method of equilibrium with HPLC had the low binding power of trimetazidine to plasma protein with high sensitivity,good reproduction,simple management and fulfilling the require-ment of the biological sample analysis.

关 键 词：曲美他嗪 血浆蛋白结合率 平衡透析法 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]