6种鳗鲡(Anguilla)线粒体DNA COⅠ序列的比较研究  

COMPARATIVE STUDY ON MtDNA COⅠGENE FRAGMENT OF SIX ANGUILLA

作　　者：吴宁[1,2] 黎中宝[1,2] 林小云[1,2] 张志红[1,2] 田鑫江[1,2] 陈晓姣[1,2] 陈丽娜[1,2] 

机构地区：[1]集美大学水产学院,厦门361021 [2]集美大学水产生物技术研究所,厦门361021

出　　处：《海洋与湖沼》2010年第6期930-934,共5页Oceanologia Et Limnologia Sinica

基　　金：公益性行业(农业)科研专项经费项目;"鳗鲡种质鉴定与新品种引进";nyhyzx07-043-08号;集美大学创新团队建设项目;2010A004号

摘　　要：采用基因测序的方法,对6种鳗鲡的COⅠ基因片段进行了PCR扩增和测序。结果表明,6种鳗鲡COⅠ基因片段的长度都为652bp,4种碱基组成非常相似,并且A+T的含量都大于G+C的含量。6种鳗鲡COⅠ基因片段核苷酸序列之间有105bp的差异,其中有18处发生碱基颠换,87处发生碱基转换。非洲鳗鲡和欧洲鳗鲡COⅠ基因片段核苷酸序列差异最大为54bp,同源性为91.72%,而美洲鳗鲡与欧洲鳗鲡COⅠ基因片段核苷酸序列差异最小为24bp,同源性为96.32%。MtDNA COⅠgene fragments of six Anguilla species were amplified by PCR, and the PCR products were purified and sequenced. The results showed that all the MtDNA COⅠgene fragments were 652bp for nucleotide sequence with similar base composition and the content of A＋T were more than that of G＋C. The base difference among six Anguilla species was 105bp, including 18 base transversions and 87 base transitions. The base difference between Anguilla mossambica and A. anguilla was the most in homology of 91.72%, however the base difference between A. rostrata and A. anguilla was the lost in homology of 96.32%.

关 键 词：鳗鲡 COⅠ基因 序列差异 颠换 转换 

分 类 号：Q75[生物学—分子生物学]