反相高效液相色谱法测定大鼠血浆中姜黄素含量的方法改进  

Content determination of curcumin in rat plasma with Improved HPLC method

作　　者：黄秀旺[1] 吴国华[1] 温彩霞[1] 邓艳平[1] 许建华[1] 

机构地区：[1]福建医科大学药学院临床药理研究所,福州350004

出　　处：《海峡药学》2010年第12期54-57,共4页Strait Pharmaceutical Journal

基　　金：福建省社会发展重点项目(2009Y0025);福建医科大学重大科研项目计划(09ZD006)

摘　　要：目的采用改进的萃取剂用于血浆的预处理,HPLC法测定姜黄素的血药浓度。方法以乙酸乙酯与二甲基亚砜(14:1,V/V)作为萃取溶剂,一次萃取血浆样品。色谱柱为XDB C18柱(150×4.6mm,5μm),流动相为乙腈-5%冰乙酸(45:55),流速1.0mL.min-1,柱温30℃,检测波长428nm,进样量10.0μL。结果采用新型萃取剂处理血浆样品,萃取回收率高,操作简单,姜黄素血药浓度在0.02～5.0μg.mL-1范围内与峰面积的线性关系良好,回归方程Y=111.71X-3.1788,r=0.9998,日内、日间精密度RSD符合生物样品的分析要求。结论本方法简便、快捷、准确、重现性好,可用于姜黄素血药浓度测定及药代动力学研究。OBJECTIVE To determine the content of curcumin in rat plasma by HPLC with simple sample preparation.METHDOS Ethylacetate and DMSO（14∶1,V/V）was used as extracting solvent to prepare plasma only one time.Curcumin was separated well on a agilent XDB C18 column（4.6mm×250mm,5μm）under 30℃.The mobile phase was acetonitrile：5% acetic acid solution（45∶55）at a flow rate of 1.0mL·min-1 and the detection wavelength at 428nm.RESULTS With this new extractant,liquid-liquid extraction method adopted to treat the plasma samples had the highest recovery rate.Between 0.02 and 5.0μg·mL-1,the method had a good linear relationship,while the regression equation was Y=111.71X-3.1788,r=0.9998;the within-day and between-days precision RSD were less than 15%;the average method recovery was more than 90%.CONCLUSION The HPLC method for the determination of curcumin in rat plasma is accurate and reliable,which could be used in pre-clinic pharmacokinetics study.

关 键 词：姜黄素 含量测定 高效液相色谱法 血浆 

分 类 号：R285.5[医药卫生—中药学]