新型双嵌段磷酸胆碱基共聚物作为siRNA基因运输载体研究  

Study on phosphorylcholine diblock copolymers as siRNA gene carriers

作　　者：郝湛军[1] 孙甜甜[2] 徐晤[1] 王志荣[1] 张卓琦[1] 曹希传[2] 

机构地区：[1]徐州医学院附属医院心内科 [2]中国矿业大学材料科学与工程学院

出　　处：《齐齐哈尔医学院学报》2010年第23期3699-3701,共3页Journal of Qiqihar Medical University

基　　金：国家自然科学基金(No.30800219;No.30670557);江苏省高校自然科学基金(No.08KJD320013);徐州医学院院长专项人才基金(No.07KJZ26)

摘　　要：将载体MPC30-DEA70(N)与siRNA(P)在N/P比值不同的条件下复合,用DNA凝胶电泳法,倒置显微镜,MTT法表征复合物溶液;同时分析胎牛血清(FBS)对细胞转染的影响。结果显示在无胎牛血清条件下siRNA能与载体MPC30—DEA70电性中和,N/P比值越大中和的越多,而有胎牛血清的条件下siRNA几乎不能与载体MPC30-DEA70电性中和;倒置显微镜观察到siRNA分子能进入细胞,转染效率随着N/P比值增大而增大;同时随着载体浓度增大,对细胞的增殖抑制性增强。以上结果显示,新型阳离子磷酸胆碱聚合物MPC30-DEA70可以有效负载和运输siRNA,是一种安全有效的非病毒类转基因载体。MPC30—DEA70/siRNA complexes were formed at different N/P ratios（cationic/anionic） conditions.The complexes were studied using gel electrophoresis,FCM,and MTT assay.Results showed some polyplexes are neutralized in the absence of FBS,significantly with the increasing of N/P ratios.Confocal laser scanning microscopy found the transfected siRNA could enter into the cells,suggesting its effective nuclear localization and the transfection efficiency were significantly enhanced with increasing of N/P ratios.These results indicate that Novel cationic phosphorylcholine copolymer can load and transport siRNA,which may act as an efficient and safe non—viral gene transfer vector.

关 键 词：MPC30-DEA70 SIRNA 转染 基因治疗 

分 类 号：R459.9[医药卫生—治疗学]