一株抗MRSA红树林真菌的分离和鉴定  

Isolation and identification of an Anti-MRSA Mangrove Fungus

作　　者：缪承杜[1,2] 林海鹏[1] 张志华 洪葵[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]广州市微生物研究所,广东广州510250 [3]忻州职业技术学院医学系微生物教研组,山西忻州036500

出　　处：《热带作物学报》2010年第11期2015-2019,共5页Chinese Journal of Tropical Crops

基　　金：科技部社会公益项目(2004DIB3J072)资助

摘　　要：红树林是重要的海洋生态系统,从海南文昌红树林保护区的红树林样品中进行真菌选择性分离,对其发酵液进行抗耐甲氧西林金黄色葡萄球菌(MRSA)活性筛选,并对活性较好的菌株进行鉴定。利用琼脂孔阱扩散法进行抗MRSA活性菌株筛选,通过培养特征、形态特征、ITS rDNA序列分析等方法对筛选得到的菌株进行鉴定。结果表明,从红树林中分离共得到608株真菌,活性筛选得到7株真菌,其中菌株092008具有较强的抗MRSA活性,通过各种指标鉴定菌株092008为黄曲霉(Aspergillus flavus)。Mangrove is an important marine ecosystem.Fungi from mangrove in Wenchang were isolated and screend by their anti-MRSA activity.The anti-MRSA activity was assayed by agar diffusion method.The active strains were identified by culture characteristics,morphological characteristics,ITS rDNA sequence and phylogenetic analysis.A total of 608 fungi were isolated from the tissue of mangrove.Seven strains showed anti-MRSA activity.One strain numbered 092008 pocessed strong anti^MRSA activity.The identification study indicated that strain 092008 was Aspergillus flavus

关 键 词：红树林 耐甲氧西林金黄色葡萄球菌(MRSA) 真菌 

分 类 号：S43[农业科学—农业昆虫与害虫防治] Q939.5[农业科学—植物保护]