中耳胆脂瘤中肿瘤抑制基因PTEN表达及其临床意义  

Expression of the tumor suppressor gene PTEN in human middle ear cholesteatoma and its clinical significance

作　　者：殷团芳[1] 刘伟[1] 李莉华[1] 杜芬[1] 任基浩[1] 谢鼎华[1] 卢永德[1] 

机构地区：[1]中南大学湘雅二医院耳鼻咽喉头颈外科,湖南长沙410011

出　　处：《中国现代医学杂志》2010年第24期3737-3740,共4页China Journal of Modern Medicine

摘　　要：目的探讨中耳胆脂瘤上皮中肿瘤抑制基因PTEN表达及其在中耳胆脂瘤上皮细胞存活机制中的作用。方法采用免疫组织化学法(SP法)检测中耳胆脂瘤组织和正常外耳道皮肤中PTEN表达。结果 PTEN主要表达于胆脂瘤和外耳道皮肤组织的上皮细胞核中,且中耳胆脂瘤上皮组织中PTEN阳性表达率明显低于正常外耳道皮肤中的表达(P<0.01)。结论 PTEN异常表达可能参与了胆脂瘤的发生过程。胆脂瘤上皮中PTEN低表达可能参与了胆脂瘤上皮细胞过度增殖机制。【Objectives】To investigate the expression of the tumor suppressor gene PTEN in human middle ear cholesteatoma epithelium,and to explore its roles in cellular survival mechanisms involved in cholesteatoma epithelial cells.【Method】The expression of PTEN in human middle ear cholesteatoma tissue and normal external ear canal skin specimen was examined by immunohistochemistry method（SP method）.【Result】PTEN expressed in the nuclei of epithelial cells of cholesteatoma and external ear canal skin,where as PTEN expression was significantly lower in cholesteatoma when compared with external ear canal skin（P 0.01）.【Conclusion】The abnormal expression of PTEN may participate in the genesis of middle ear cholesteatoma.The lower expression of PTEN suggest that cellular survival mechanisms may be involved in cholesteatoma epithelial hyperplasia.

关 键 词：中耳 胆脂瘤 肿瘤抑制基因 PTEN 免疫组织化学 

分 类 号：R764.21[医药卫生—耳鼻咽喉科]