维吾尔药材刺山柑种子组培快繁研究  

Tissue Culture and Rapid Propogation of Seeds of Uyghur Traditional Herbal Capparis spinosa

作　　者：马生军[1] 陆婷[2] 张爱勤[3] 王颖[1] 周琳[1] 

机构地区：[1]新疆农业大学药学院,新疆乌鲁木齐830052 [2]新疆农业大学林学和园艺学院,新疆乌鲁木齐830052 [3]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《中药材》2010年第12期1833-1836,共4页Journal of Chinese Medicinal Materials

基　　金：新疆自然科学基金项目(200821105)

摘　　要：目的:研究维吾尔药材刺山柑的组培快繁技术,为大量生产种苗提供方法和技术。方法:以新疆吐鲁番采集的刺山柑种子为实验材料,以MS为基本培养基,通过不同的激素种类和浓度配比,选择出最佳培养基配方。结果:最佳消毒条件为流水冲洗8 h,升汞灭菌12 min。最佳启动和继代培养基为MS+6-BA 0.6 mg/L+NAA 0.1mg/L,最佳增殖培养基为MS+6-BA0.6 mg/L+2,4-D 1.0 mg/L,最佳生根培养基为MS+IBA 0.8 mg/L+活性炭300 mg/L。结论:本研究得出的方法可用于工厂化生产维吾尔药材刺山柑种苗。Objective：To optimize the tissue culture and rapid proliferation techniques of seeds of Capparis spinosa for producing large scale seedlings.Methods： The seeds of Capparis spinosa were collected as explants and cultivated in different MS media,which were from Turpan in Xinjiang.The optimum media were selected by adjusting the combinations of different hormone and concentration.Results： The best on institution of asepsis explants was rinsing for 8 hours and 0.1% HgCl2 for 12 minutes.The medium MS＋6-BA 0.6 mg/L＋NAA 0.1 mg/L was suitable for primary and second culture.The medium MS＋6-BA 0.6 mg/L＋2,4-D 1.0 mg/L was suitable for proliferation,and the optimum medium of rooting was MS＋IBA 0.8 mg / L＋300 mg/L activated carbon.Conclusion： The rapid proliferation technique of seeds of Capparis spinosa can be used for producing large scale seedlings.

关 键 词：刺山柑 种子 组培 种苗 

分 类 号：R282.2[医药卫生—中药学]