药用石斛ISSR分子标记研究  

Identification of Medical Dendrobium Herbs by ISSR Marker

作　　者：杨立昌[1] 邓辉[1] 乙引[1] 张习敏[1] 王亚筑[1] 林俊清[1] 

机构地区：[1]贵州师范大学生命科学院,贵州贵阳550001

出　　处：《中药材》2010年第12期1841-1844,共4页Journal of Chinese Medicinal Materials

基　　金：贵州省教育厅科研基金重点项目(2007-018);国家科技支撑计划项目(2009BAI74B02-3);贵州省科技重大专项计划(2008-6022);贵州省科技创新人才团队建设项目(2009-4007)

摘　　要：目的:探索药用石斛鉴别的分子生物学方法,为药用石斛质量控制提供理论依据。方法:采用ISSR-PCR方法扩增药用石斛DNA片段,根据基因分型能力筛选ISSR引物,并建立药用石斛鉴别条形码。结果:筛选出两条Rp值大于8的引物,能完全区分6个种共8个类群的药用石斛。结论:ISSR分子标记可用于药用石斛的种类和产地鉴定。Objective：A molecular biology method was studied to identify medical Dendrobium and to provide a method for quality control of these plants.Method： ISSR primer was screened through ISSR-PCR reaction according to its gene resolving power,and digital barcodes were established for identification.Results：Screening 2 primers which Rp value above 8,This primers can identify medical Dendrobium from 6 kinds 8 groups.Conclusion： ISSR molecular maker technology is useful for identifying species and habitats of medical Dendrobium plants.

关 键 词：ISSR-PCR 分子标记 药用石斛 基因分型能力 鉴别 

分 类 号：R282.71[医药卫生—中药学]