大孔吸附树脂法及离子交换层析柱法精制纯化丹参多糖的研究  

Study on Purification of Polysaccharides in Root of Salvia miltiorrhiza by Macroporous Adsorption Resin and Ion-exchange Resin Methods

作　　者：汤伟[1] 彭求贤[1] 严愉妙[1] 莫志贤[1] 黄少慧[1] 李欣[2] 蔡红兵[1] 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]南方医科大学肿瘤研究所,广东广州510515

出　　处：《中药材》2010年第12期1937-1941,共5页Journal of Chinese Medicinal Materials

基　　金：广东省科技计划项目(20090315);白云区科技计划项目(2009-SZ-42);中国博士后科学基金(20090450199)

摘　　要：目的:对中药丹参中多糖成分进行提取、分离、精制和纯化研究。方法:丹参药材经过水提醇沉得到粗多糖(SMP)。采用大孔树脂法及离子交换层析柱法精制纯化粗多糖,综合比较D-101、AB-8、DB-301及MG-1等4种大孔吸附树脂对粗多糖的分离纯化效果,进一步以DEAE-52离子交换层析法对过AB-8柱制备得到的粗多糖进行分离精制。结果:粗多糖中总多糖含量为40.35%,蛋白质含量为8.96%,经4种大孔树脂初步纯化后发现AB-8、DB-301型的树脂效率高,纯化后多糖含量分别可达78.72%、80.45%。总多糖经DEAE-52离子交换树脂进一步分离得到SMP1、SMP2、SMP3三个组分。结论:AB-8、DB-301型大孔吸附树脂及DEAE-52型树脂可应用于丹参多糖的精制纯化。Objective：To study the purification and isolation of polysaccharides from Salvia miltiorrhiza.Methods： The root was extracted by water purified preliminarily by alcohol precipitation,and then four different types macroporous adsorption resin and one ion-exchange resin were comparatively investigated in the purification and isolation of Salvia polysaccharides.Results： The total polysaccharides of crude extracts was 40.35%,and protein content amounted to 8.96%.Compared with the traditional methods,AB-8,DB-301 type of resin used in purification of polysaccharides could simplify the working process and obtain better effect.Then the obtained crude polysaccharides through AB-8 resin were purified by ion-exchange resin DEAE-52.Three portions of powder were obtained through lyophilization and named as SMP1,SMP2,SMP3.Conclusion： Purification of Salvia polysaccharides can be conducted by adoping AB-8,DB-301 type of resin and DEAE-52 ion-exchange resin.

关 键 词：多糖 含量测定 分离纯化 大孔吸附树脂 离子交换层析 

分 类 号：R284.2[医药卫生—中药学]