正交法优化异株百里香挥发油提取及β-环糊精包合工艺研究  

Studies on the Extraction of Volatile Oil from Thymus marschallianus and Preparation of Its β-CD Inclusion Compounds

作　　者：张海英[1] 李伟[1] 

机构地区：[1]新疆医科大学附属中医医院,新疆乌鲁木齐830000

出　　处：《中药材》2010年第12期1942-1946,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:考察异株百里香挥发油的最佳提取工艺和β-环糊精最佳包合工艺。方法:采用单因素及L9(34)正交试验设计,以挥发油得率作为评价指标优选异株百里香挥发油提取工艺;以包合物收得率和挥发油包合率的综合评分作为评价指标优选β-CD包合工艺。结果:挥发油最佳提取工艺为加水12倍量,浸泡0.5 h,提取4 h;挥发油的最佳包合工艺为采用饱和水溶液法,挥发油与β-环糊精的比例为1∶8,包合温度为60℃,包合时间为60 min。结论:所选提取工艺提取率高,包合工艺合理,节约成本。Objective：To study the optimum conditions for the extraction of volatile oil from Thymus marschallianus and the preparation of its β-CD inclusion compounds.Methods：The study was carried out with single factor and orthogonal design.With the percentages of extracted volatile oil of Thymus marschallianus as the target marker we selected the optimum conditions for extracting the volatile oil.The ration of inclusion and the oiling-bearing from the inclusion as the target marker was to optimize the conditions for including the oils with β-CD.Results：The optimized extraction conditions were as follows： the water was 12 times of the herbs in weight,Thymus marschallianus should be extracted for 4 hours after soaked 0.5 hours with water.The optimized inclusion conditions were as follows： applying the saturated aqueous solution method,the proportion of oil and β-CD was 1mL∶8g,the temperature and time were 60 ℃ and 60 minutes,respectively.Conclusion：The established process can be used for the extraction and the inclusion of volatile oil from Thymus marschallianus with its high ration of oil and the economic cost.

关 键 词：异株百里香 挥发油提取 Β-环糊精包合物 单因素试验 正交试验 

分 类 号：R283.6[医药卫生—中药学]