木荷树皮杀虫活性成分追踪的初步试验  

Tracing Insecticidal Ingredients from Barks of Shima superba Gardn. et Champ.

作　　者：邓志勇[1] 邓业成[2] 周国永[1] 陈兴田[1] 

机构地区：[1]贺州学院化学与生物工程系,广西贺州542800 [2]广西师范大学生命科学学院,广西桂林541004

出　　处：《农药》2010年第8期612-614,共3页Agrochemicals

基　　金：广西高校重点建设实验室"桂东特色资源开发与利用研究实验室"研究项目(2009SYS[2]12);贺州学院院级科研项目(2009KYYB07)

摘　　要：采用活性追踪和化学分离相结合的方法对木荷树皮甲醇提取物中的活性成分进行分离,用石油醚、氯仿和正丁醇连续萃取,经生物活性测定,正丁醇萃取物的活性最大。木荷树皮正丁醇萃取物在2 g/L质量浓度下,对小菜蛾3龄幼虫24、48 h的拒食率分别为91.30%和92.44%,拒食中浓度(AFC50)分别为0.457 0、0.434 6 g/L;对菜青虫4龄幼虫,在1 g/L质量浓度下,24、48 h的拒食率分别为99.08%和96.44%,拒食中浓度(AFC50)分别为0.041 9、0.055 7 g/L。Methanol extracts from barks of Schima superba Gardn.et Champ.（L.） were isolated by means of phytochemistry combined with bioassay.Methanol extracts were continuously extracted by petroleum ether chloroform and n-butanol,and the n-butanol extract possessed the highest activity,with the 24 and 48 h antifeedant rates against 3rd instar of Plutella xylostella（L.） being 91.30 and 92.44% at 2 g/L,respectively,and 24 and 48 h antifeedant median concentration（AFC50） 0.4570 and 0.4346 g/L.with the 24 and 48 h antifeedant rates against 4th instar of Pieries rapae（L.） being 99.08 and 96.44% at 1 g/L,respectively,and 24 and 48 h antifeedant median concentration（AFC50） 0.0419 and 0.0557 g/L.

关 键 词：木荷 小菜蛾 菜青虫 拒食活性 

分 类 号：S482.3[农业科学—农药学]