太行黑山羊FGF5基因CDs区的序列特征及其表达规律研究  

Study on sequence characterization and expression pattern of Taihang black goat FGF5 CDs region

作　　者：何永新[1] 陈玉林[1] 姜维[1] 薛鹏[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2010年第12期45-50,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：农业部转基因生物新品种培育科技重大专项"高产绒量转基因绒山羊新品种培育"(2008ZX08008-002)

摘　　要：【目的】克隆太行黑山羊成纤维细胞生长因子5(FGF5)基因CDs区,并对其进行序列特征分析和实时荧光定量表达检测。【方法】于2009年的7～10月份采集太行黑山羊的皮肤组织样品,利用RT-PCR方法克隆FGF5基因CDs区序列,并分析其序列特征及在不同生长发育时期的表达情况。【结果】太行黑山羊FGF5基因CDs区全序列长度为813bp,编码270个氨基酸,其中包含一个FGF结构域(87～221位氨基酸);太行黑山羊FGF5基因CDs区核苷酸序列与牛、马、人、猩猩和小鼠的同源性分别为98%,92%,90%,90%和86%,其编码氨基酸序列与牛、马、人、猩猩和小鼠的同源性分别为98%,91%,91%,91%和91%;实时荧光定量分析结果表明,在检测的4个月份的皮肤组织中,FGF5基因在9月份表达量显著高于7、8、10月份(P<0.05),而7、8、10月份之间表达量无显著差异(P>0.05)。【结论】太行黑山羊的FGF5基因编码的氨基酸高度保守;太行黑山羊毛囊发育在7、8月份处于生长期,9月份由生长期向退行期过渡,10月份进入退行期。[Objective] The CDs region of Taihang black goat Fibroblast Growth Factor5（FGF5） gene was cloned and the sequence characterization and quantitative analysis were performed.[Method] The skin tissue of Taihang black goat was collected in July,August,September and October.The CDs region was cloned from total RNA by RT-PCR.The sequence characterization and expression of follicle growth in different development periods of FGF5 gene were performed.[Result] The CDs region of FGF5 in Taihang black goat consisted of 813 nucleotides encoding 270 amino acids with a FGF domain from 87-221 amino acids.The nucleotides sequence homology of FGF5 CDs of Taihang black goat was found to be 98%,92%,90%,90% and 86% compared with that of bovine,horse,human,orangutanand mouse,while the amino acid sequence homology was 98%,91%,91%,91% and 91% respectively;The result of real-time quantitative PCR showed：the FGF5 was highly expressed in September,significantly higher than July,August and October（P〈0.05）,but there was no significant difference between these three months（P〈0.05）.[Conclusion] The amino acids coded by the gene FGF5 are highly conserved in Taihang black goat.The result of real-time quantitative indicated that the follicle development of Taihang black goat is in anagen in July and August,followed by the transition of anagen and catagen in September,and catagen in October.

关 键 词：太行黑山羊 FGF5基因 RT-PCR 基因序列 氨基酸序列 

分 类 号：S826[农业科学—畜牧学] Q636[农业科学—畜牧兽医]