普通小麦-华山新麦草矮秆种质B62的分子细胞学鉴定  

Molecular cytology on a dwarf germplasm derived from Triticum aestivum×Psathyrostachys huashanica

作　　者：武军[1] 马琳[1] 赵继新[1] 陈新宏[1] 刘淑会[1] 杨群慧[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2010年第12期123-127,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学科研专项(Z109021002);西北农林科技大学唐仲英育种基金项目(A212020716);陕西省重大科技攻关"13115"专项(2007ZDKG-01)

摘　　要：【目的】对普通小麦(Triticum aestivum L.)品种7182与华山新麦草(Psathyrostac hyshuashanica)杂交后代中选育的矮秆种质B62进行农艺性状考察和外源遗传物质检测。【方法】以普通小麦-华山新麦草矮秆种质B62为材料,在田间农艺性状考察的基础上,采用细胞学及基因组原位杂交(根尖与花粉母细胞中期Ⅰ)技术,检测B62中的华山新麦草遗传物质。【结果】根尖细胞学鉴定表明,B62染色体条数为44条;根尖细胞染色体原位杂交(GISH)表明,B62携带2条华山新麦草染色体;花粉母细胞减数分裂Ⅰ中期染色体GISH结果显示,2条外源染色体能够配对,说明2条异源染色体是华山新麦草的1对同源染色体。【结论】矮秆种质B62为普通小麦-华山新麦草二体异附加系,其农艺性状优于其小麦亲本7182。[Objective] The study was to observe the agronomy and alien genetic chromatin of a dwarf germplasm B62 obtained by screening the offspring of hybridizing between Triticum aestivum L.cv.7182 and Psathyrostachys huashanica.[Method] Cytology analysis,genomic in situ hybridization（GISH） analysis on root tip and PMC Ⅰ of B62 were used.[Result] 44 chromosomes were observed after identificating root tip in cytology.With P.huashanica Ns genomic DNA as a probe,root tip genomic in situ hybridization（GISH） analysis identified two material carries P.huashanica chromosomes.Then it was concluded that B62 was a wheat-P.huashanica disomic or double monosomic addition line;and the addition line for pollen mother cell mid-meiosisⅠgenomic in situ hybridization（GISH）.The results show that the two alien chromosomes can pair and arrange in equatorial plate.It shows that the two added chromosomes are a pair of homologous chromosomes in P.huashanica.[Conclusion] B62 is a dwarf material of the wheat-P.huashanica hybridization disomic addition line,which agronomic are traits better than the wheat parent-7182.

关 键 词：普通小麦 华山新麦草 矮秆 异附加系 GISH分析 

分 类 号：S512.101[农业科学—作物学]