时辰对罗哌卡因、氯普鲁卡因和利多卡因惊厥半数有效量和半数致死量的影响  

Effects of chrono on median lethal dose and median effective dose of convulsion induced by Ropivacaine,Chloroprocaine and Lidocaine

作　　者：陆苏[1] 戴体俊[2] 宗雨[1] 张纯洁[1] 李勇[3] 刘莹[1] 徐佳[3] 李奕青[1] 徐小林[4] 孟晶[2] 

机构地区：[1]徐州医学院麻醉学院,江苏221002 [2]徐州医学院麻醉药理学教研室,江苏221002 [3]徐州医学院临床学院,江苏221002 [4]徐州医学院公共卫生学院,江苏221002

出　　处：《上海医学》2010年第10期919-921,共3页Shanghai Medical Journal

基　　金：国家自然科学基金资助课题(30872432;30471657;39970715);江苏省自然科学基金资助课题(BK2001143)

摘　　要：目的探讨时辰对罗哌卡因、氯普鲁卡因、利多卡因惊厥半数有效量(ED50)和半数致死量(LD50)的影响。方法 270只小鼠随机分为ED50和LD50两组,每组135只;每组再分为3个亚组,即罗哌卡因亚组、氯普鲁卡因亚组、利多卡因亚组,每亚组45只;按不同时辰每亚组再随机分为3个次亚组,每次亚组15只,分别于8:00、14:00和20:00腹腔注射罗哌卡因、氯普鲁卡因或利多卡因,以小鼠惊厥和死亡为观察指标,用序贯法测定各时间点的ED50及LD50。结果利多卡因在14:00的惊厥ED50显著低于8:00和20:00(P值分别<0.05、0.01),在14:00的LD50显著低于8:00(P<0.05),其余各时辰间ED50、LD50的差异均无统计学意义(P值均>0.05)。罗哌卡因在8:00的惊厥ED50和LD50均显著低于14:00和20:00(P值分别<0.05、0.01),其余各时辰间ED50、LD50的差异均无统计学意义(P值均>0.05)。氯普鲁卡因在8:00的ED50显著低于14:00(P<0.05),其余各时辰间ED50、LD50的差异均无统计学意义(P值均>0.05)。结论罗哌卡因、氯普鲁卡因和利多卡因的惊厥ED50均具有时辰依赖性。利多卡因、罗哌卡因的LD50具有时辰依赖性,氯普鲁卡因的LD50未发现时辰依赖性。Objective To observe the effects of chrono on the median lethal dose（LD50）and the median effective dose（ED50）of convulsion induced by anaesthetics（Ropivacaine,Chloroprocaine,and Lidocaine）.Methods A total of 270 mice were evenly randomized into 2 groups for investigation of LD50 and ED50;the two groups were then further assigned to 3 subgroups,with each subgroup including 45 mice;and each subgroup was also evenly divided into 3 tertiary groups.Ropivacaine,Chloroprocaine and Lidocaine were intraperitoneally injected at 8：00,14：00 and 20：00,respectively.The convulsion and death of animals were observed.And the ED50 and LD50 values were determined by up-down sequential allocation at different time points.Results The convulsive ED50 of Lidocaine at 14：00 was significantly lower than those at 8：00 and 20：00（P0.05,0.01,respectively）.The LD50 value of Lidocaine at 14：00 was significantly lower than that at 8：00（P0.05）.The convulsive ED50 and LD50 values of Ropivacaine at 8：00 were significantly lower than those at 14：00 and 20：00（P0.05,0.01,respectively）.The convulsive ED50 value of Chloroprocaine at 8：00 was lower than that at 14：00（P0.05）.Chrono showed no significant effect on the LD50 values of Chloroprocaine.Conclusion The effects of convulsive ED50 values of Ropivacaine,Chloroprocaine and Lidocaine are chrono-dependent.The effects of LD50 values of Ropivacaine and Lidocaine also are chrono-dependent.The effects of LD50value of Chloroprocaine is not chrono-dependent.

关 键 词：时辰药理学 局部麻醉药 惊厥 半数有效量 半数致死量 

分 类 号：R614[医药卫生—麻醉学]