原子转移自由基聚合法制备强阴离子交换色谱填料及其在蛋白质分离中的应用  

Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation

作　　者：王婀娜[1] 段淑静[1] 何锡文[1] 陈朗星[1] 李文友[1] 张玉奎[1,2] 

机构地区：[1]南开大学化学学院,天津300071 [2]中国科学院大连化学物理研究所,大连116011

出　　处：《化学学报》2010年第23期2421-2428,共8页Acta Chimica Sinica

基　　金：国家重点基础研究发展规划(973计划;No.2007CB914100);国家自然科学基金(Nos.20875049;20875050)资助项目

摘　　要：在大孔硅胶表面引入原子转移自由基聚合的引发基团,通过该技术将甲基丙烯酰氧乙基三甲基氯化铵聚合于硅胶表面,制得了强阴离子交换色谱填料.详细考察了该填料对标准蛋白质的分离性能及流动相pH、流速、有机溶剂对蛋白质保留的影响.实验结果表明,在流速为0.75 mL/min时,采用线性梯度洗脱,20 min内可快速分离不同体系的三种标准蛋白;并将该填料用于鸡蛋中卵清蛋白的快速分离纯化,取得了很好的效果.A new strong anion-exchange stationary phase for HPLC has been synthesized by surface-initiated atom transfer radical polymerization（ATRP）.Firstly,2-bromoisobutyryl bromide as the ATRP initiator was attached onto the macroporous silica via chemical reaction.In the next stage,methacrylatoethyl trimethyl ammonium chloride（MATA） carrying strong anion exchanger groups as the monomer was po-lymerized on the initiator-immobilized particles via surface-initiated ATRP.After studying the adsorption properties,the suitable support was packed into stainless steel column to separate acid proteins by the linear gradient elution in 20 min at flow rate of 0.75 mL/min.The effects of flow rate,pH and organic solvents on the separation and retention ability of proteins were studied in detail.This support was also used for the separation and purification of ovalbumin from egg in only one step.

关 键 词：硅胶 强阴离子交换色谱 原子转移自由基聚合 蛋白质分离 

分 类 号：Q51-3[生物学—生物化学]