反复电针对佐剂性关节炎大鼠伏膈核尾状核内大麻素CB1受体表达的影响  

EFFECTS OF REPEATED ELECTRO-ACUPUNCTURE ON PROTEIN AND GENE EXPRESSION OF CANNABINOID CB1 RECEPTOR IN NUCLEUS ACCUMBENS AND CAUDATE NUCLEUS IN INFLAMMATORY-PAIN RATS

作　　者：寿崟[1] 赵颖倩[1] 徐鸣曙[2] 葛林宝 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]上海市针灸经络研究所脑神经生物实验室,上海200030 [3]上海市气功研究所,上海200030 [4]上海市针灸经络研究中心,上海201203

出　　处：《中国疼痛医学杂志》2010年第6期348-352,共5页Chinese Journal of Pain Medicine

基　　金：国家重点基础研究发展计划(973计划)(2009CB522901);上海市科学技术委员会科研计划项目课题基金(07DZ19722-5)资助课题

摘　　要：目的:研究大麻素CB1受体是否参与电针镇痛。方法:以完全弗氏佐剂造成AA大鼠模型,在电针治疗四次后以大麻素CB1受体拮抗剂AM251进行干预,检测各组大鼠缩爪反射潜伏期(PWL)的变化,并应用实时荧光定量PCR(FQ-PCR)和Western blot检测伏膈核、尾状核内CB1受体mRNA和蛋白质表达情况。结果:(1)电针治疗后大鼠PWL延长,电针+AM251组的电针镇痛效果显著弱于电针组(P<0.01);(2)电针+AM251组较电针组大鼠伏膈核、尾状核内CB1受体mRNA表达水平显著降低(P<0.01),但与假模型组和模型组比较,无显著性差异(P>0.05)。(3)Western blot结果与FQ-PCR结果一致:电针+AM251组较电针组大鼠伏膈核、尾状核内CB1受体蛋白质表达水平显著降低(P<0.01)。但与假模型组和模型组比较,无显著性差异(P>0.05)。结论:AM251能够翻转电针后大鼠伏膈核、尾状核内CB1受体的高表达,减弱电针镇痛作用,提示CB1受体可能参与电针镇痛作用。Objective： To explore the relationship between cannabinoid CB1 receptor and repeated electro-acupuncture（EA） analgesia.Methods： In the complete Freund＇s adjuvant-induced arthritis rats,paw withdrawing latency was investigated after repeated EA or the injection of cannabinoid CB1 receptor selective antagonist AM251.And the CB1 receptor mRNA was measured by using Real time fluorescence quantitative-polymerase chain reaction（FQ-PCR）.The Western blot was used to observe the changes of CB1 receptor protein expression.Results：（1） The paw withdrawing latency was prolonged after the repeated EA while the analgesic effects of EA were attenuated after the injection of AM251（P〈0.01）.（2） In the nucleus accumbens and caudate nucleus,the expression of CB1 receptor mRNA of EA＋AM251 group was significantly decreased than that of repeated EA group（P〈0.01）.There was no significant difference of gene expression of CB1 receptor between EA＋AM251 group and sham model group or model group.（3）Western blot demonstrated the similar result as FQ-PCR： the CB1 receptor protein expression of EA＋AM251 group was notably decreased than that of repeated EA group（P〈0.01）. There was no significant difference of protein expression of CB1 receptor between EA＋AM251 group and sham model group or model group.Conclusion： AM251 can attenuate the analgesic effects of repeated EA by reversing CB1 receptor higher expression,suggesting that CB1 receptor may play an important role in the EA analgesia.

关 键 词：电针 佐剂性关节炎 大麻素受体-1 大麻素受体-1拮抗剂 

分 类 号：R245.97[医药卫生—针灸推拿学]