栽培与野生川西獐牙菜有效成分的研究  

Comparison Study on active ingredcent on the Artificial and Wild Swertia mussotii Franch

作　　者：拉本[1,2] 陈志[1,2] 马永贵[1,2] 

机构地区：[1]青海师范大学青藏高原环境与资源教育部重点实验室 [2]青海师范大学生命与地理科学学院,西宁810008

出　　处：《青海师范大学学报（自然科学版）》2010年第4期57-60,共4页Journal of Qinghai Normal University(Natural Science Edition)

摘　　要：目的:测定野生与栽培川西獐牙菜中3种有效药用成分,即龙胆苦苷、芒果甙和齐墩果酸的含量.方法:采用高效液相色谱法分离和测定川西獐牙菜中龙胆苦苷、芒果甙和齐墩果酸的含量.流动相:甲醇-水-0.5%乙酸(25∶75∶0.8)、甲醇-0.5%乙酸(25∶75)和甲醇-水(90∶10);检测波长:270nm、254nm和215nm;流速:1.0mL/min;进样量:10μL.结果:栽培川西獐牙菜中龙胆苦苷、芒果甙和齐墩果酸含量与野生种基本相同.结论:栽培川西獐牙菜完全可以代替野生种.Aim： Three kinds of effectively medical component such as oleaolic acid,mangiferin and gentiopicroside of artificial and wild Swertia mussotii Franch were determined.Method： The content of oleaolic acid,mangiferin and gentiopicroside of Swertia mussotii Franch were separated and determined by HPLC.The conditions of HPLC were as follows： mobile phase,methanol-water-0.5（25：75：0.8）,methanol-0.5（25：75） and methanol-water（90∶10）;detectived wave length,270nm,254nm and 215nm;flowing rate,1.0mL/min;injection volume,10μL.Result： The content of oleaolic acid,mangiferin and gentiopicroside of wild and artificial Swertia mussotii Franch was the same.Conclusion： The wild Swertia mussotii Franch was completely replaced by the artificial one.

关 键 词：HPLC 川西獐牙菜 龙胆苦苷 芒果甙 齐墩果酸 

分 类 号：R927.2[医药卫生—药学]