甜椒CaHSP22.5基因的克隆及序列分析  

Isolation and Sequence Analysis of CaHSP22.5 Gene in Sweet Pepper

作　　者：贺立龙[1] 郭尚敬[1] 冯海霞[1] 王洪霞[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《聊城大学学报（自然科学版）》2010年第4期74-78,共5页Journal of Liaocheng University:Natural Science Edition

基　　金：国家自然科学基金资助项目(30671242);山东省优秀中青年科学奖励基金资助项目(2008BS07008)

摘　　要：为深入研究小分子量热激蛋白对植物胁迫条件下的保护机理,利用RT-PCR结合RACE的方法,克隆甜椒内质网小分子量热激蛋白基因(CaHSP22.5)基因并进行序列分析和转基因烟草分析.获得了包括全长开放读码框(ORF)的CaHSP22.5基因的cDNA序列,与目前已克隆的ERsHSP类基因的同源性分析表明,甜椒CaHSP22.5基因编码的蛋白与马铃薯和番茄的ERsHSP类蛋白的同源性最高.通过叶盘法利用农杆菌介导转化烟草,成功获得了正反义转基因烟草,为进一步研究该基因的功能和作用机制奠定了基础.The study provides a theoretical basis for further study of protection mechanisms by small heat shock proteins（sHSP） under stress conditions.Methods of RT-PCR and RACE were used for cloning CaHSP22.5 gene from sweet pepper.The cDNA of CaHSP22.5 containing of the full-length open reading frame（ORF） was islated from sweet pepper（Capsicum annuum L.）.The alignment analysis of the deduced CaHSP22.5 protein with other known ERsHSP family proteins indicated that CaHSP22.5 is highly homologous with potato StC119 and tomato LeHSP21.3.In addition,Method of leaf disc was used for transforming tobacco mediated by Agrobacterium.Sense and antisense transgenic tobacco plants were obtained.This study lay the foundation for further study the mechanism of CaHSP22.5 gene.

关 键 词：甜椒 内质网 小分量热激蛋白 CaHSP22.5 基因克隆 

分 类 号：Q74[生物学—分子生物学]