大口鳒线粒体DNA控制区结构和鲽形目鱼类的系统进化初步研究  

Characterization of mitochondrial control region sequence of Psettodes erumei and phylogenetic analysis of Pleuronectiformes

作　　者：张艳春[1] 孔晓瑜[2] 王忠明[2] 

机构地区：[1]中国海洋大学教育部海水养殖研究重点实验室,山东青岛266003 [2]中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室/热带海洋生物标本馆,广东广州510301

出　　处：《热带海洋学报》2010年第6期71-78,共8页Journal of Tropical Oceanography

基　　金：国家自然科学基金项目(30870283)

摘　　要：测定大口鳒Psettodes erumei线粒体控制区全序列,并与其他7种鲽形目鱼类控制区结构进行比较分析。结果表明,大口鳒线粒体控制区全长大约1601bp,5’端和3’端分别存在56bp和8bp的串联重复序列。除此之外,其控制区结构和其他种类相似,分为终止相关序列区(TAS)、中央保守区(CSB-D、CSB-E、CSB-F)、保守序列区(CSB-1、CSB-2、CSB-3),同时在CSB-D后还识别出鲽形目保守的Poly-T结构。比较了8种鲽形目鱼类控制区全序列和去除重复序列后控制区序列的碱基组成变化,结果显示前者的种间差异明显大于后者,变异系数约是后者的两倍。这种差异主要是由于重复序列的异质性如长度及其拷贝数不同、碱基组成不同所造成的。基于控制区部分序列对鲽形目12种鱼类进行系统分析,结果显示,在鲽形目内部大口鳒分类地位最原始;鲽亚目先与鳎亚目聚在一起,后与鳒亚目聚在一起,这个结果与基于形态特征的系统关系相符。Complete control region（CR） of Psettodes erumei mitochondrial genome was determined.Its size is 1,601 bp in length,with a 56 bp tandem repeat at 5＇ end and a 8 bp one at 3＇ end.Based on the alignment of CR sequences in this species and seven other flatfishes,three domains（the termination associated sequence domain,TAS;the central conserved domains,CSB-F,CSB-E,CSB-D and the conserved sequence block domains,CSB-1,CSB-2,CSB-3） and a Poly-T tract are identified.The difference of base composition of complete CR sequences is greater than that of CR sequence without repeated regions among eight flatfishes,and the variation coefficient of the former is about twice of the latter,which is mainly due to the het-erogenicity as length,base composition and copy numbers of repeated regions.With Carangoides armatus as the outgroup,the molecular phylogenetic relationship of 12 flatfishes was analyzed using the ML and BI methods.The results showed that P.erumei is the most primitive species among the flatfishes.Soleoidei and Pleuronectoidei are clustered into one clade,and then Psettodoidei merges to it.These results are consistent with that based on the morphological analyses.

关 键 词：线粒体DNA 控制区 大口鳒Psettodes erumei 系统发生 鲽形目 

分 类 号：Q178.53[生物学—水生生物学] Q953[生物学—普通生物学]