斜带石斑鱼烂尾病病原菌的分离与鉴定  

Isolation and identification of bacteria pathogens from Epinephelus coioides with tail-rotted disease

作　　者：梅冰[1] 周永灿[1] 徐先栋[1] 王世峰[1] 谢珍玉[1] 

机构地区：[1]海南大学海洋学院,海南海口570228

出　　处：《热带海洋学报》2010年第6期118-124,共7页Journal of Tropical Oceanography

基　　金：农业科技成果转化项目(2009GB2E200302);国家自然科学基金项目(30660144;30760190;31060360);海南省自然科学基金项目(80617);海南省教育厅高等学校科研项目(Hj200729)

摘　　要：2008年5月从海南省三亚红沙港与陵水新村湾网箱养殖的患"烂尾病"的斜带石斑鱼Epinephelus coioides中分离到3株优势菌株HS08001、HS08002和XC08001。人工感染试验表明,这3株菌株为斜带石斑鱼的致病菌。经菌株形态特征、培养特性和生理生化反应等表型测定以及16S rDNA测序分析,这3株细菌被鉴定为哈维氏弧菌Vibrio harveyi。其中,HS08001对斜带石斑鱼的半致死剂量为1.05×104CFU.g-1鱼体重。药敏试验表明,该菌具有较强的耐药性,在所检测的25中抗菌药物中,仅对庆大霉素、氯霉素和利福平高度敏感,而对阿莫西林等18种抗菌药物不敏感。A very serious tail-rotted disease broken out in the cage-cultured Epinephelus coioides in Sanya city and Lingshui County,Hainan province,China in May 2008.Three strains of HS08001,HS08002,and XC08001 were isolated from the dis-eased grouper and were shown to be the pathogens by artificial infection.All the three isolates were identified as Vibrio har-veyi by biochemical tests and 16S rDNA sequence analysis.The LD50 value of strain HS08001 for E.coioides was 1.05×104 CFU/g body weight.Sensitivity test to 25 chemotherapeutic agents indicated that the pathogen was only highly sensitive to gentamicin,chloramphenicol,and rifampicin.

关 键 词：斜带石斑鱼Epinephelus coioides 哈维氏弧菌Vibrio HARVEYI 烂尾病 16SrDNA 

分 类 号：S941.42[农业科学—水产养殖]