激素诱导花鳗鲡精巢发育成熟期间性激素水平及相关细胞的超显微结构  

Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata

作　　者：齐鑫[1] 黄海[1,2] 周雯伊[1] 尹绍武[1] 张勇[2] 刘晓春[2] 陈国华[1] 林浩然[1,2] 

机构地区：[1]海南大学海洋学院暨热带生物资源教育部重点实验室,海南海口570228 [2]中山大学水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室,广东广州510275

出　　处：《热带海洋学报》2010年第6期130-136,共7页Journal of Tropical Oceanography

基　　金：国家自然基金面上项目(30770283);"十一五"国家科技支撑计划重点项目(2007BAD29B03);海南省教育厅高等学校科学研究资助项目(Hjkj2008-22);广东省科技兴海(渔)项目(B200701A06)

摘　　要：通过注射人类绒毛膜促性腺激素(HCG)诱导雄性花鳗鲡性腺发育成熟,每7d注射一次(剂量为500U.(体重)kg-1),共注射6次。检测此期间血清中促性腺激素(GtH)和性类固醇激素(T、11-KT、E2)的水平,以及相关细胞超显微结构的变化。结果表明,对照组雄性花鳗鲡性腺发育处于Ⅰ―Ⅱ期,GS(IGonadosome Index)为0.17±0.06%,血清中GtH、T、11-KT水平较低,并且T浓度低于0.1ng(试剂盒测定范围为0.1―20ng.mL-1);注射第3次后,实验组鱼性腺发育处于Ⅲ期,GSI为7.0±0.03%,血清中GtH、T、11-KT的水平均明显上升;排精后的实验组鱼GSI为9.57±2.1%,GtH、T、11-KT的水平比注射第3次的实验组鱼有所下降,但仍然高于对照组;在整个催熟过程中,血清中E2水平持续降低,从对照组的2.36ng.mL-1下降到排精后的0.83ng.mL-1。超显微结构的观察证明,与对照组相比排精后的实验组鱼脑垂体中GtH细胞的小颗粒减少,内质网池扩大,出现分泌并释放促性腺激素后留下的空泡。对照组鱼肝细胞富含糖原,几乎没有脂肪泡的存在,细胞核位于细胞中间,排精后的实验组鱼肝细胞脂肪泡增多变大,脂肪泡的形成导致细胞核偏位。Serum levels of gonadotropin（GtH）,testosterone（T）,11-ketotestosterone（11-KT）,and estradiol-17β（E2） were assessed by radioimmunoassay during induction of sexual maturation of male marbled eel（Anguilla marmorata） by Human Chorionic Gonadotropin（HCG） treatment.Morphological alterations of the liver and GtH cell of Anguilla marmorata were investigated by means of light and electron microscopy.The testis Gonadosome Index（GSI） was 0.17±0.06% in the control group and their serum GtH and 11-KT levels were low,the T level was lower than 0.1 ng（the range of kit was 0.1―20ng·mL-1）;Serum GtH,T,11-KT levels increased rapidly in the operated group after the third injection of HCG,when the GSI of testis was 7.0±0.03%.After the spermiation,the GtH,T and 11-KT levels decreased a little,but still higher than the levels of the control group.Serum E2 levels decreased gradually as HCG treatment progressed,from 2.36 ng/ml of the control group to 0.83 ng/ml of the spermiation eels.In the hepat of the control group,abundant glycongen and little lipid droplets were obversived,the nuclear was in the middle of the cell.In contrast,in the hepat of the spermiation eel,the lipid droplets accu-mulated,which lead to the closing of nuclear to the cell membrane.

关 键 词：花鳗鲡Anguilla marmorata 人工繁殖 性类固醇激素 促性腺激素 精子 GtH细胞 肝细胞 

分 类 号：Q492.4[生物学—生理学]