12种中药有效成分对3株水产致病菌体外抑菌试验  

Antibacterial Experiment of 12 Chinese herbs active ingredient on 3 bacteria from the Aquatic Products in Vitro

作　　者：聂鲡蓉[1] 郑曙明[1] 仇登高[1] 

机构地区：[1]西南大学鱼类繁育与健康养殖中心,重庆402460

出　　处：《重庆水产》2010年第3期17-20,共4页CHONGQING FISHERIES

基　　金：科技部科技人员服务企业行动项目（2009GJF10007）资助.

摘　　要：选择12种有抑菌作用的中药的有效成分，采用平板挖洞法对3种水产常见致病菌作药物敏感试验，然后采用二倍稀释法测定12种中药有效成分对3种致病菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。结果表明：12种中药有效成分对3株细菌都有不同程度的抑制作用，其中没食子酸、槲皮素、黄岑昔、大黄酸、盐酸小檗碱、大黄素甲醚对温和气单胞菌抑制效果最好，没食子酸、槲皮素对温和气单胞菌MIC，MBC最小，MIC为0．03125mg／ml，MBC为0．0625mg／ml：大黄素甲醚、没食子酸、甘草酸、槲皮素对嗜水气单胞菌抑制效果最好，大黄素甲醚、没食子酸、甘草酸对嗜水气单胞菌MIC，MBC最小，MIC为0．03125mg／ml，MBC为0．0625mg／ml；大黄素甲醚、片草酸、没食子酸、盐酸小檗碱对变形杆菌抑制效果为最好，没食子酸，人黄素甲醚、甘草酸对变形杆菌MIC，MBC均最小，MIC为0．03125mg／ml，MBC为0．0625mg／ml。Sensitive test of these bacteria were carried out by dig a hole in plate. Then double dilution method was used for MIC and MBC test. Results： The compositions of 12 Chinese herbs active ingredient can inhibit the growth of 3 bacteria in different levels,garlic acid, quercetin, baicalin, chrysophanic, berberine acid and rheochrysidin have the best effects on Aeromonas sobria, garlic acid and quercetin have the smallest MIC, MBC, MIC 0.03125mg/ml,MBC O.0625mg/ml 1;Rheochrysidin, garlic acid , glycyrrhizi cacid and quercetin have the best effects on Aeromonas hydrophila, rheochrysidin, garlic acid, glycyrrhizic acid have the smallest MIC, MBC, MIC 0.03125mg/ml, MBC 0.0625mg/ml 1;Physcion, Rheochrysidin, Glycyrrhizic acid, garlic acid and berberine acid have the best effects on Proteus vulgaris, rheochrysidin, Glycyrrhizic acid have the smallest MIC, MBC, MIC 0.03125mg/ml,MBC 0.0625mg/ml.

关 键 词：中药有效成分 温和气单胞菌 嗜水气单胞菌 变形杆菌 抑菌圈 最小抑菌浓度 最小杀菌浓度 

分 类 号：S853.74[农业科学—临床兽医学]