葛根素对急性高眼压后大鼠视网膜Bcl-2和Bax蛋白表达的影响  

Effects of Kakkonein on the Protein Expression of Bcl-2 and Bax in Rat Retina after Acute Injury

作　　者：周文学[1] 张军富[2] 陈翠[3] 

机构地区：[1]嘉鱼县人民医院眼科,湖北嘉鱼437200 [2]潍坊市人民医院眼科 [3]潍坊市益都中心医院眼科

出　　处：《咸宁学院学报（医学版）》2010年第6期470-472,共3页Journal of Xianning Univarsity(medical Sciences)

摘　　要：目的观察急性高眼压后大鼠视网膜Bcl-2和Bax蛋白表达变化及葛根素干预的影响。方法以免疫组化法检测急性高眼压后大鼠视网膜不同时间点Bcl-2和Bax蛋白表达。结果 A组(对照组)视网膜神经节细胞层可见一定的Bcl-2和Bax蛋白表达量;B组(加压组)Bcl-2蛋白表达在2h开始升高,36h达高峰,72h时恢复正常,其中12h到48h各时间点与A组比较差异有统计学意义(P<0.01),Bax蛋白的表达变化与Bcl-2相近,不同的是达高峰时间点在24h。C组(加压+葛根素组)12h至48h与B组相应时间点比较,Bcl-2蛋白的表达上升,差异有统计学意义(P<0.05),Bax蛋白的表达下降,与B组比较差异有统计学意义(P<0.05)。结论急性高眼压后大鼠视网膜Bcl-2和Bax蛋白表达均升高,葛根素上调了Bcl-2蛋白表达,下调了Bax蛋白表达。Objective To study the effects of kakkonein on the protein expression of Bcl-2 and Bax in rat retina after acute intraocular hypertension.Methods After acute intraocular hypertension,changes in protein expression of Bcl-2 and Bax were detected by immunohistochemical method.Results In group A,there is positive expression of Bcl-2 and Bax protein.In group B,the level of positive expression of Bcl-2 and Bax protein was increased from 12h to 48h,and there is statistical difference as compared to group A（P﹤0.01）.There was statistical difference on the expression of Bcl-2 and Bax between Group B and C from 12h to 48h（P﹤0.05）.Conclusion The protein expression of Bcl-2 and Bax was increased in rat retina after acute intraocular hypertension and kakkonein could upregulate Bcl-2 expression and downregulate Bax expression.

关 键 词：急性高眼压 BCL-2蛋白 BAX蛋白 葛根素 

分 类 号：R965.1[医药卫生—药理学]