吸烟对慢性阻塞性肺疾病脂质过氧化系统及α_1-抗胰蛋白酶的影响  

Effects of Smoking on Lipid Peroxides Antioxidetion and α_1-Antitrypsin in COPD

作　　者：牛秀英[1] 梁燕生[1] 王金良[1] 

机构地区：[1]天津市公安医院,300050

出　　处：《天津医药》1999年第11期655-657,共3页Tianjin Medical Journal

摘　　要：研究吸烟对慢性阻塞性肺疾病（ＣＯＰＤ）患者体内自由基水平和α_１－抗胰蛋白酶（α_１－ＡＴ）的影响。采用硫代巴比妥法、放射免疫法和酶标法对３５例吸烟和２５例非吸烟ＣＯＰＤ患者血清脂质过氧化物（ＬＰＯ）、超氧化物歧化酶（ＳＯＤ）、α_１－ＡＴ和α_１－酸性糖蛋白（α_１－ＡＧ）进行测定。结果显示：吸烟ＣＤＰＤ组血中ＬＰＯ、α_１－ＡＧ明显高于非吸烟组．ＳＯＤ、α_１－ＡＴ明显低于非吸烟组；吸烟组ＬＰＯ和α_１－ＡＴ呈负相关（ｒ＝－０．４７１，Ｐ＜０．０１），ＳＯＤ和α_１－ＡＴ呈正相关（ｒ＝０．４６７，Ｐ＜０．０１）。结论：慢性阻塞性肺疾病存在慢性炎症，氧化作用强，抗氧化能力弱，α_１－ＡＴ活性减低。吸烟影响机体的抗氧化能力，影响肺的蛋白酶／抗蛋白酶平衡，对ＣＯＰＤ的发生、发展有重要作用。Detecting free radicals, α_l -antitrypsin(α_1-AT)and α_1 acid glucoprotein(α_1-AG) from smoder with smoking- induced diseases. Method:35 smokers and 25 non-smokers with COPD as the study group,25 healthy non-smokers as the control group, the metabolic thiobarbituric acid method(TBA), RIA and enzyme-linked immunosorbent assay were used to detect the lipid peroxides(LPO), superoxide dismutase(SOD), al -AT and α_1-AG. Results: In the blood of smokers with COPD group LPO,α_1-AG were significantly higher than non-smokers with COPD group,but the SOD and α_1-AT contents, . were significantly lower. There was a good positive correlation between SOD and α_1-AT in smokers with COPD(r = 0. 467, P<0 .01 ),but a significant negative correlaion between LPO ang α_1 -AT(r = -0 .471, P<0.01 ). Conclusion:COPD have chronic inflammation, the increase of oxidation and decrease of antioxidation activity of α_1-AT and unbalance of protease and antiprotease might play an important role in smoking-induced diseases.

关 键 词：吸烟 阻塞性肺疾病 抗胰蛋白酶 过氧化脂质 

分 类 号：R563.902[医药卫生—呼吸系统]