RP-HPLC法测定升麻药材中升麻苷H-1含量  

Determinating the Content of Cimifugoside H-1 in Cimicifugae Rhizome by RP-HPLC

作　　者：靳波[1,2] 刘友平[1,2] 陈鸿平[1,2] 赵祎姗[1,2] 彭月[1,2] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《四川中医》2011年第3期66-68,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：质检公益性行业科研专项(No.2007GYB075-2);校学生科研实践创新课题(013-011-39)

摘　　要：目的：从升麻中分离得到升麻苷H-1并建立其定量测定方法。方法：采用硅胶柱色谱从升麻乙醇提取液的水不溶性部位分离得到升麻苷H-1;采用HPLC法,色谱柱：Hypersil BDS C18柱（200mm×4.6mm,5μm）,流动相：甲醇-水（60∶40）,检测波长：210nm,流速：1.0ml/min,柱温：30℃。结果：升麻苷-1在0.358~6.444mg范围内呈现良好的线性关系（r=0.9996）,精密度、稳定性和重现性的RSD均小于2%,平均加样回收率为101.9%,RSD为4.81%。结论：该方法准确、快速、重现性好,可用于测定升麻药材中升麻苷H-1的含量。Objective：To establish an accurate method for the determination of Cimicifugoside H-1which was isolated from Cimicifugae Rhizome.Methods：Cimicifugoside H-1 was isolated from water-insoluble part of Cimicifuga ethanol extract by silica gel column chromatography;HPLC analysis was performed on Hypersil BDS C18 column（200mm×4.6mm,5μm）with a mobile phase consisting of methanol：water（60∶40）.The flow rate was 1.0ml/min.The detection wavelength was 210 nm and the column temperature was 30℃.Results：The linear range of Cimicifugoside H-1 was within 0.358~6.444 mg（r=0.9996）.The results that RSD of precision,stability,reproducibility were all less than 2%.The average recovery was 101.9% and RSD was 4.81%.Conclusions：The method is simple and accurate and can be used for determinating Cimicifugoside H-1 of Cimicifugae.

关 键 词：升麻 升麻苷H-1 RP-HPLC 

分 类 号：R284.2[医药卫生—中药学]