p90rsk-1在绵羊卵母细胞体外成熟过程中的表达  

Expression of p90rsk-1 in the ovine oocytes during in vitro maturation

作　　者：白瑞霞[1,2] 曹贵方[1] 赵鹏伟[1] 

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018 [2]内蒙古医学院基础医学院,呼和浩特010018

出　　处：《内蒙古大学学报（自然科学版）》2011年第2期211-214,共4页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金项目(30660128)

摘　　要：采用Western blotting检测p90rsk-1在绵羊卵母细胞体外成熟过程中的表达.实验分别在0h、8h、16h、24h检测绵羊卵母细胞p90rsk-1的表达量的变化,并进行统计学分析.结果p90rsk-1在绵羊卵母细胞体外成熟过程中均为阳性表达.随着成熟时间的延长,卵母细胞p90rsk-1在16h的相对表达量显著升高(P<0.05),但在24h又下降.因此推测Rsk在绵羊卵母细胞体外成熟过程中起一定的作用.The expression of p90rsk-1 was detected by western blotting during in vitro maturation of ovine oocytes.The expression was measured at 0h,8h,16h and 24h of maturation,respectively,and the data were analyzed.The results showed that the p90rsk-1 protein of ovine oocytes was expressed during the whole maturation period.The expression level of p90rsk-1 at 16h of maturation is significantly higher than that of the others.It was suggested that Rsk play somewhat a role in vitro maturation of ovine oocytes.

关 键 词：p90rsk-1 绵羊卵母细胞 体外成熟 WESTERN BLOTTING 

分 类 号：Q813.7[生物学—生物工程]