用于HRP测定的一种主动式PDMS微混合器  

Investigation of an Active PDMS Micromixer for Horseradish Peroxidase Detection

作　　者：詹志坤[1,2] 童兆宏[1,3] 曲艳丽[1] 董再励[1,3] SRINIV ASAN Balaji 

机构地区：[1]中国科学院机器人学国家重点实验室,沈阳110016 [2]中国科学院研究生院,北京100084 [3]美国阿肯色州立大学机械工程系,费耶特维尔市72701

出　　处：《纳米技术与精密工程》2011年第2期134-139,共6页Nanotechnology and Precision Engineering

基　　金：国家高技术研究发展计划(863计划)资助项目(2009AA04Z313);机器人学国家重点实验室自主课题资助项目(RLZ200808);中国科学院;国家外国专家局创新团队国际合作伙伴计划资助项目

摘　　要：微米尺度下的生物流体混合是生物微机电系统(BioMEMS)和分析化学等领域研究的重要问题之一,目前的混合方式多以被动式自由扩散为主,存在流体混合效率不高的现象.针对该问题,设计了一种主动式聚二甲基硅氧烷(PDMS)微混合器.该装置利用脉冲气体对多个PDMS微隔膜进行顺序驱动,可实现微混合室中不同液体的快速混合和反应.同时,基于鲁米诺-过氧化氢体系的化学发光反应机理,以辣根过氧化物酶(HRP)作为催化剂,进行了微混合器最佳工作条件研究,基于HRP化学发光分析法和L25-5-6正交实验,确定出微混合器化学发光分析法的最优实验条件组合,并在此实验条件下完成了HRP的微量测定.实验结果表明,目前检测体系下线性范围为2.05×10-9 mol/L～2.05×10-8 mol/L,检测限为2.05×10-9 mol/L,检测反应时间小于2 min,可满足生物医学和分析化学领域对于物质测定方面的微量、快速的检测要求.Mixing of bio-fluids on the micro-scale is an important issue in biomicroelectro-mechanical systems（BioMEMS） and analytical chemistry,and presently the passive diffusion is the main way in BioMEMS with a low mixing efficiency.An active micromixer based on polydimethylsiloxane（PDMS） was porposed in this paper.A control circuit actuated an electromagnetic valve（EMV） to produce a series of compressed air pulses that deflected the PDMS diaphragms of the six air chambers in a sequence,facilitating the mixing and reactions in the mixing chamber.Based on the chemiluminescent mechanism of the luminol-H2O2 system catalyzed by horseradish peroxidase（HRP）,the optimal working conditions of the micromixer were studied and the optimal experiment conditions were established with chemiluminescencent analytical method for HRP detection and L25-5-6 orthogonal experiment.HRP detection on the micro scale was conducted at the optimal experimental conditions,results of which show that the linear range and detection limit of HRP are 2.05×10-9 mol/L-2.05×10-8 mol/L and 2.05×10-9 mol/L,respectively,while the total reaction time of chemiluminescence is less than 2 min.Therefore,the proposed micromixer can satisfy the demand for micro scale and quick detection of materials in the field of biomedicine and analytical chemistry.

关 键 词：主动式微混合器 化学发光 辣根过氧化物酶 微量测定 

分 类 号：Q503[生物学—生物化学]