生长因子和雌激素对大鼠垂体前叶细胞增殖活性及催乳素基因表达的影响  

Effects of Growth Factors and Estrogen on the Proliferation and Prolactin Gene Expression in Anterior Pituitary Cells of Rats

作　　者：吴雪梅[1] 张荣[1] 狄安稞[1] 单惠敏[1] 黄曼影[1] 许荣焜[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所生理研究室,北京100005

出　　处：《中国医学科学院学报》1999年第5期331-337,共7页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金!39770287和39870341;卫生部科学基金!98-1-007

摘　　要：目的观察外源性生长因子和雌激素对离体正常大鼠垂体前叶细胞增殖活性及催乳素（ｐｒｏｌａｃｔｉｎ，ＰＲＬ）基因表达的影响，并探讨生长因子与雌激素作用的关系。方法应用无血清原代培养的大鼠垂体前叶细胞，分别采用荧光染色细胞ＤＮＡ的激光扫描共聚焦显微镜（ｌａｓｅｒｓｃａｎｎｉｎｇｃｏｎｆｏｃａｌｍｉｃｒｏｓｃｏｐｙ，ＬＳＣＭ）扫描、以及原位杂交方法，观察１７－β－雌二醇（ｅｓｔｒａｄｉｏｌ，Ｅ２）、表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈ　ｆａｃ－ｔｏｒ，ＥＧＦ）及转化生长因子β１（ｔｒａｎｓｆｏｒｍｉｎｇｇｒｏｗｔｈｆａｃｔｏｒβ１，ＴＧＦβ１）对大鼠垂体前叶细胞增殖活性和催乳素（ｐｒｏｌａｃｔｉｎ，ＰＲＬ）基因表达的影响。结果生长因子或雌激素作用３６ｈ后，Ｅ２和ＥＧＦ单独作用组细胞ＤＮＡ含量和ＰＲＬｍＲＮＡ含量均较正常对照组明显升高（Ｐ＜０．００１）；两者共同作用上述效应更为显著，高于Ｅ２和ＥＧＦ单独作用组（Ｐ＜０．０１）。ＴＧＦβ１对细胞增殖与ＰＲＬ基因表达均有抑制作用（Ｐ＜０．００１）；而与Ｅ２共同作用时细胞内ＤＮＡ含量和ＰＲＬｍＲＮＡ水平显著高于ＴＧＦβ１单独作用组（Ｐ＜０．００１），但仍低于Ｅ２单独作?Detect the effects of exogenous 17 B-estradiol (E2),epidermal growth factor (EGF),and transforming growth factor B1 (TGFB1 ) on the proliferation and prolactin(PRL) gene expression in primary serum-free cultured anterior pituitary cells in vitro. Methods Laser scanning con focal microscopy (LSCM ) and in situ hybridization in primaryserum-free cultures of rat anterior pituitary cells were employed. Results After 36 hours incubation of the monolayer with E2(10 -8 mol/L) and EGF (10-8 mol/L),DNA and PRL mRNA contents in the cells were increased significantly (P<0. 001 );and when cells were co-incubated with E2 and EGF at the same time,　the levels of DNA and PRL mRNA were higherthan those treated with E, or EGF alone (P<0.01 ), respectively. TGFB1 (2 ng/ml ) treatment decreased the DNA and PRL mRNA contents significantly (P<0. 001 ). Its inhibitoryeffect was reduced at the presence of E2,the DNA and PRL mRNA levels in the cells werehigher than those treated with TGFB1 alone (P<0. 001),but still lower than E2 alone treatment (P<0. 001). Conclusions The results indicate that EGF and TGFB1 exerte stimulatory inhibitory effects,　on cell proliferation and PRL gene expression in anterior pituitary cellsof rats in both hasal and E2-induced conditions. EGF and TGFB1 may be involoved in the regulation of proliferation and PRL gene expression in anterior pituitary cells in vivo; and alsomay be correlated with prolactin-secreting tumors formation induced by E2.

关 键 词：垂体前叶细胞 生长因子 雌二醇 增殖活性 催乳素 

分 类 号：Q453[生物学—生理学]