正常国人乳果糖和甘露醇排出率比值  

Excretion Ratio of Lactulose and Mannitol as intestinal Permeability Index in Healthy Chinese Volunteers

作　　者：刘卫[1] 蒋朱明[1] 舒红[1] 孙梅 李德敏[1] 刘跃武[1] 邓高月[1] 

机构地区：[1]中国医学科学院中国协和医科大学协和医院外科,北京100730 [2]卫生部国际交流中心

出　　处：《中国医学科学院学报》1999年第5期406-411,共6页Acta Academiae Medicinae Sinicae

基　　金：卫生部重点项目!97010204

摘　　要：目的测定正常国人口服乳果糖、甘露醇测试液后尿中乳果糖／甘露醇排出率比值（Ｌ／Ｍ）。方法用带脉冲电化学检测器的高压液相仪（ＨＰＬＣ－ＰＥＤ）分别测定６０例健康志愿者口服以往剂量（乳果糖１０ｇ、甘露醇５ｇ）和小剂量（乳果糖２ｇ、甘露醇１ｇ）测试液后，尿中乳果糖和甘露醇浓度，并计算乳果糖和甘露醇尿中排出率及其比值。结果口服以往剂量和小剂量测试液６ｈ后，正常国人尿中Ｌ／Ｍ比值分别为０．０２６±０．００６和０．０３６±０．００８。结论ＨＰＬＣ－ＰＥＤ方法检测灵敏度高，可减少乳果糖和甘露醇的测试量，测定乳果糖与甘露醇排出率比值反映肠粘膜通透性是一种实用的临床方法。To determine lactulose (L)/mannitol (M) excretion ratio with high pressureliquid chromatograph with pulsed electrochemical detection (HPLC-PED) in healthy Chinesevolunteers. Methods Sixty healthy Chinese volunteers enrolled. For each volunteer the L/M permeability study was performed twice by two different doses, as small dose (1 gmannitol and 2 g lactulose) and regular dose (5 g mannitol and 10 g lactulose). The urinewere collected during 6 hours after ingestion of the test solution. The M and Lconcentrations were analyzed by HPLC with pulsed electrochemical detection. Results TheL/M ratio was 0. 026±0. 006 in the small doses tests, and 0. 036±0. 008 in the large dosestests. Conclusions With HPLC-PED detection, a low doses of lactulose and mannitol canbe used to study intestinal permeability. Determination of the L/M ratio may be a way ofevaluating intestinal barrier function in clinical practice.

关 键 词：肠粘膜通透性 乳果糖 甘露醇 排出率比值 

分 类 号：R322.45[医药卫生—人体解剖和组织胚胎学] R977.6[医药卫生—基础医学]