内脂素通过上调ACAT1诱导THP-1源性泡沫细胞形成  

visfatin induces THP-1-derived foam cell formation by up-regulating the expression of ACAT1

作　　者：康静[1] 成蓓[1] 姜蕾[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院高干科,武汉430022

出　　处：《中国免疫学杂志》2011年第3期213-216,共4页Chinese Journal of Immunology

摘　　要：目的：观察酰基辅酶A：胆固醇酰基转移酶1（ACAT1）在内脂素（visfatin）诱导THP-1源性泡沫细胞形成中的作用,以初步探讨visfatin诱导泡沫细胞形成的分子机制。方法：THP-1单核细胞诱导分化为巨噬细胞,予不同浓度的visfatin（1×10-71×10-5mol/L）分别作用048小时,运用油红O染色观察细胞浆脂滴变化,酶荧光法检测细胞内总胆固醇（TC）和游离胆固醇（FC）含量,TC与FC之差为胆固醇酯（CE）含量。分别运用RT-PCR和Western blot检测ACAT1 mRNA和蛋白表达。结果：与对照组比较,visfatin干预组细胞浆脂滴明显增多,CE与TC的比值明显增加（〉50%）。visfatin呈浓度和时间依赖性地上调ACAT1 mRNA和蛋白表达（P〈0.05）。结论：ACAT1表达上调是visfatin诱导THP-1源性泡沫细胞形成的机制之一,这可能为visfatin致动脉粥样硬化发病机制的研究提供一个新的理论依据。Objective：To investigate the role of acyl-CoA：cholesterol acyltransferase 1（ACAT1） in THP-1-derived foam cell formation induced by visfatin,and to discuss the mechanism of visfatin-induced foam cell formation.Methods：THP-1 monocytes were induced into macrophages by 160 nmol/L phorbol myristate acetate（PMA）for 48 h,then the macrophages were added with visfatin（1×10-7mol/L-1×10-5 mol/L）for 0 to 48 h.Lipid droplets in cytoplasm were observed by Oil red O staining.The contents of intracellular total cholesterol（TC） and free cholesterol（FC） were detected by enzyme fluorescence analysis.The contents of cholesterol ester（CE） were calculated by the difference between TC and FC.The expressions of ACAT1 mRNA and protein were determined by RT-PCR and Western blot respectively.Results：The lipid droplets of THP-1-derived macrophages were increased by visfatin and the ratios of CE to TC were much higher than 50%.The expression of ACAT1 mRNA and protein was up-regulated by visfatin in both concentration-and time-dependent manner（P〈0.05）.Conclusion：The up-regulation of ACAT1 expression is involved in THP-1-derived foam cell formation induced by visfatin.This may provide a new evidence for visfatin inducing atherosclerosis.

关 键 词：内脂素 酰基辅酶A 胆固醇酰基转移酶1 泡沫细胞 动脉粥样硬化 

分 类 号：R543[医药卫生—心血管疾病]