丹酚酸B抑制人鼻咽癌细胞生长的体外试验研究  

Suppression of Growth of Human Nasopharyngeal Carcinoma Cells by Salvianolic Acid B in Vitro

作　　者：刘雄[1] 唐纯志[2] 王路[1] 林少雄[1] 万仁强[1] 陈静[3] 

机构地区：[1]南方医科大学南方医院耳鼻咽喉头颈外科,广东广州510515 [2]广州中医药大学针灸推拿学院,广东广州510405 [3]南方医科大学中医药学院,广东广州510515

出　　处：《广州中医药大学学报》2011年第2期163-166,218,共5页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】研究丹酚酸B(Sal B)对体外培养的人低分化鼻咽癌细胞株C666-1生长的抑制作用及可能的机制。【方法】采用四甲基偶氮唑盐(MTT)法检测Sal B对C666-1细胞的半数抑制剂量(IC50),以IC50浓度的Sal B处理C666-1细胞48 h后,采用Hoechst 33258进行凋亡染色,荧光显微镜下计数凋亡细胞,流式细胞仪检测细胞周期,观察Sal B对C666-1细胞周期及凋亡的影响。【结果】经Sal B处理24、48、72 h后,C666-1细胞生长受到抑制,以48 h最为显著,IC50为(80±6.8)μg/mL;流式细胞仪检测结果显示Sal B可使C666-1细胞G1期显著延长(P<0.001),凋亡染色计数显示Sal B可诱导C666-1细胞凋亡(P<0.001)。【结论】Sal B体外抑制人鼻咽癌细胞C666-1生长的作用与其能使C666-1细胞G1期显著延长,促进细胞凋亡有关。Objective To investigate the suppression of human nasopharyngeal carcinoma cell line C666-1 by salvianolic acid B（Sal B） in vitro and to explore its initial mechanism.Methods Fifty percent inhibiting concentration（IC50） of Sal B for cell line C666-1 was detected with methyl thiazolyl tetrazolium（MTT） assay.After treated with Sal B at IC50 for 48 hours,apoptotic cells were dyed with Hoechst 33258 and then were counted under fluorescence microscope,and cell cycle was detected by flow cytometry.Results After treated with Sal B for 24,48 and 72 hours,the growth of cell line C666-1 was inhibited,and the inhibition was obvious after treatment for 48 hours.IC50 of Sal B was about（80±6.8） μg/mL.The results of flow cytometry showed a significantly higher percentage of G1 phase in C666-1（P〈0.001）.The results of counting under fluorescence microscope showed that Sal B induced apoptosis of C666-1（P〈0.001）.Conclusion The mechanism of Sal B for inhibiting the growth of human nasopharyngeal carcinoma cell line C666-1 in vitro is probably related with the prolongation of G1 phase and with the induction of apoptosis of C666-1.

关 键 词：丹酚酸B/药理学 鼻咽癌/中药疗法 细胞凋亡 细胞周期 细胞培养 体外的 鼻咽癌细胞/病理学 

分 类 号：R285.5[医药卫生—中药学]