内抑素组织表达趋势与肾间质微血管损伤的相关性研究  

The trend for tissue-specific expression of endostatin and its correlation with tubulointerstitial microvascular injury

作　　者：吕临静[1] 马宏[2] 郭晓媛[2] 郭茂盛[2] 李卫卫[2] 王亚娟[2] 

机构地区：[1]山西医科大学研究生院,太原030001 [2]山西医科大学第一医院儿科,太原030001

出　　处：《中国药物与临床》2011年第3期271-274,共4页Chinese Remedies & Clinics

基　　金：山西省高校科技研究开发基金(2003103)

摘　　要：目的探讨在幼年肾间质微血管损伤过程中内抑素、血管内皮生长因子(VEGF)、CD31组织定位表达的趋势、相关性及其潜在意义,以及用阿托伐他汀干预的效应。方法采用单侧输尿管结扎(UUO)模型,将90只SD雄性大鼠随机分为假手术组、模型组和阿托伐他汀治疗组(治疗组)。在术后第1,3,5,7,14天每组各取6只大鼠处死,左肾组织行Masson染色,动态观察肾脏病理学变化,并用免疫组织化学方法测定肾组织中内抑素、VEGF、CD31的表达变化。结果随着梗阻时间延长UUO模型组内抑素蛋白表达进行性增高,而VEGF及CD31蛋白表达水平却逐渐下降;与UUO模型组相比,治疗组内抑素蛋白表达减少,而VEGF及CD31蛋白表达增高。内抑素与VEGF及CD31之间呈负相关。结论在幼年大鼠肾间质微血管损伤过程中,内抑素表达上调具有重要的致病意义,而阿托伐他汀可以通过下调内抑素减轻肾间质微血管损伤。Objective To investigate the trend for tissue-specific expression,relevance and the potential significance of endostatin（ENS）,vascular endothelial growth factor（VEGF） and CD31 during renal microvascular injury in young rats,and to explore the intervention effect of atorvastatin.Methods Unilateral ureteral obstruction（UUO） models were established in SD rats.A total of 90 male SD rats were randomly divided into shame operation group,UUO model group and atorvastatin-treated group.On days 1,3,5,7 and 14 after operation,every 6 SD rats from each group were sacrificed for dynamic observation of kidney pathological changes with Masson staining in the left kidney tissue where altered expressions of ENS,VEGF and CD31 was determined subsequently by immunohistochemistry.Results Along with the increase of obstruction time,the UUO group showed progressively increased ENS expression but a gradual decline in expressions of VEGF and CD31.On the contrary,the atorvastatin-treated group had a decline in the expression of ENS but an elevation in expressions of VEGF and CD31 as compared with the UUO group.The expression of ENS was negatively correlated with those of VEGF and CD31.Conclusion In the process of tubulointerstitial microvascular injury,the up-regulated expression of ENS exhibits an important role in pathogenesis.Atorvastatin may alleviate the tubulointerstitial microvascular injury by down-regulating the expression of ENS.

关 键 词：输尿管梗阻 肾疾病 间质纤维化 内抑素 

分 类 号：R692[医药卫生—泌尿科学]