环境激素双酚A与蛋白质相互作用的电化学研究  

Electrochemical Studies of the Interaction between Environmental Hormone Bisphenol A and Protein

作　　者：王辰[1] 付艳丽[1] 王振[1] 高云涛[1] 

机构地区：[1]云南民族大学化学与生物技术学院民族药资源化学国家民委-教育部重点实验室,云南昆明650031

出　　处：《云南民族大学学报（自然科学版）》2011年第2期92-95,98,共5页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：云南省社会发展科技计划项目(2007B148M);民族药资源化学国家民委-教育部重点实验室开放基金(MJY090101)

摘　　要：采用电化学法研究环境激素双酚A与牛血清蛋白的相互作用.实验表明牛血清蛋白存在使双酚A的氧化峰峰电流减小.双酚A在加入牛血清蛋白后参与反应的质子数和电子数都为2.牛血清蛋白与双酚A的结合数m=2,结合常数β=5.81×1010.由此推测双酚A进入牛血清蛋白的疏水空腔内,使游离双酚A浓度变小.The interaction between environmental hormone bisphenol A and bovine serum albumins was investigated by the means of electrochemical method. The results indicated that the oxidation peak currents of bisphenol A decreased when bovine serum albumins was added. Both proton and electron of electrochemical oxidation of bisphenol A at the electrode were 2. The combining ratio of bisphenol A to bovine serum albumins was 2： 1, with the combining constants of 5.81 × 10^10. The proposed interactions is that BPA enters the hydrophobic water cavity of bovine serum albumins, causing the decrease of the dissociative concentration.

关 键 词：双酚A 牛血清蛋白 循环伏安法 相互作用 

分 类 号：O657.13[理学—分析化学]