ITP患者大剂量地塞米松治疗前后外周血B-1a细胞的变化  

The level change of peripheral blood B-1a cell in immune thrombocytopenia patients treated with high-dose dexamethasone

作　　者：丁柳美[1] 朱列君[1] 化范例[2] 

机构地区：[1]复旦大学附属金山医院检验科,上海200540 [2]复旦大学附属金山医院血液科,上海200540

出　　处：《检验医学》2011年第3期160-162,共3页Laboratory Medicine

摘　　要：目的研究免疫性血小板减少症(ITP)患者大剂量地塞米松治疗前后外周血B-1a细胞水平的动态变化。方法用流式细胞仪和CD5CD19双标抗体检测20名正常对照,31例ITP患者大剂量地塞米松治疗前后外周血B细胞和B-1a细胞占淋巴细胞的比例和绝对值,并比较地塞米松治疗有效和无效患者之间B-1a细胞变化的差别。结果 ITP患者治疗前外周血B-1a细胞占淋巴细胞比例为(4.01±3.32)%,绝对值为(74.8±26.6)×106/L,均较正常对照组明显升高(P<0.01),地塞米松治疗结束后ITP患者外周血B-1a细胞水平下降至(31.9±14.5)×106/L,其中地塞米松有效和无效患者治疗前B-1a细胞分别为(91.8±44.3)×106/L和(48.9±25.8)×106/L(P<0.05),治疗结束后有效和无效患者外周血B-1a细胞分别为(22.5±12.3)×106/L和(43.9±13.2)×106/L。结论 ITP患者外周血B-1a细胞水平升高,接受大剂量地塞米松治疗后,B-1a细胞绝对值明显下降,治疗前B-1a细胞水平较低者提示对大剂量地塞米松治疗反应较差。Objective To investigate the dynamic changing level of peripheral blood B-1a cell in immune thrombocytopenia（ITP） patients treated with high-dose dexamethasone.Methods Flow cytometry and CD5 CD19 antibody were used to detect the absolute counts of peripheral blood B cell and B-1a cell and their proportion in lymphocyte in 20 healthy controls and 31 patients with ITP treated with high-dose dexamethasone.The changes of B-1a cell level after treatment were analyzed between response group and non-response group.Results Before the treatment,the peripheral blood B-1a cell proportion was（4.01±3.32）% of lymphocyte in ITP patients,and the absolute count was（74.8±26.6）×106/L,which were significantly higher than those in controls（P0.01）.After the treatment,B-1a cell level decreased to（31.9±14.5）×106/L.Before the treatment,B-1a cell levels in response group and non-response group were（91.8±44.3）×106/L and（48.9±25.8）×106/L respectively（P0.05）.The count decreased to（22.5±12.3）×106/L and（43.9±13.2）×106/L after the treatment.Conclusions The level of peripheral blood B-1a cell increases in patients with ITP and decreases significantly after high-dose dexamethasone treatment.In those patients who have a relative lower B-1a cell count,the high-dose dexamethasone treatment trends to show worse efficacy.

关 键 词：B-1a细胞 免疫性血小板减少症 流式细胞术 

分 类 号：R446.11[医药卫生—诊断学]