过度训练及补充谷氨酰胺对大鼠额叶皮质中神经营养素和核转录因子水平的影响  

Impact of Overtraining and Glutamine Supplementation on Neurotrophins and Nuclear Transcroption Factor in Frontal Cortex of Rat Brain

作　　者：于动震[1] 苏彦炬[2] 董静梅[3] 陈佩杰[1] 

机构地区：[1]上海体育学院博士后流动站,上海200438 [2]河北经贸大学体育教学部,河北石家庄050061 [3]兰州城市学院体育学院,甘肃兰州730070

出　　处：《上海体育学院学报》2011年第2期52-56,共5页Journal of Shanghai University of Sport

基　　金：国家自然科学基金资助项目(30971422);上海市重点学科(第3期)建设资助项目(S30802)

摘　　要：目的:探讨过度训练及补充谷氨酰胺对大鼠额叶皮质中BDNF、NGF、NT-3及其mRNA和核转录因子p-CREB水平的影响。方法:60只SD大鼠随机分为空白对照组(C组)、过度训练组(E组)、谷氨酰胺补充组(G组),E组和G组进行递增负荷跑台训练11周,最后一次训练结束后36 h断头分离额叶皮质。应用ELISA方法检测BDNF、NGF、NT-3的蛋白含量,Real time-PCR方法检测BDNF、NGF、NT-3的mRNA转录,Western blot检测p-CREB的蛋白表达。结果:与C组比较,E组额叶皮质中BDNF和NGF蛋白含量显著升高(分别为P<0.01和P<0.05),NT-3水平显著下降(P<0.05),与E组比较,G组额叶皮质中BDNF和NGF显著下降(分别为P<0.01和P<0.05);与C组比较,E组额叶皮质中BDNF和NGF mRNA的表达水平显著升高(均为P<0.05),与E组比较,G组额叶皮质中BDNFmRNA的表达水平显著下降(P<0.05);与C组比较,E组额叶皮质中p-CREB表达水平显著升高(P<0.05),与E组比较,G组额叶皮质中p-CREB的表达水平显著降低(P<0.05)。结论:过度训练可明显影响大鼠额叶皮质中BDNF、NGF及其mRNA和p-CREB的表达水平;谷氨酰胺补充可在一定程度上逆转大鼠过度训练时额叶皮质中BDNF及其mRNA的表达水平,同时伴随p-CREB表达水平的变化。Objective： To investigate the effects of overtraining and glutamine supplementation on BDNF, NGF, NT-3, their mRNA and p-CREB in frontal cortex of rat brain. Methods： Sixty Sprague-Dawley rats were randomly divided into three groups： blank control group （ C ）, overtraining group （E） and glutamine supplementation group （G）. Group E and G were trained in standard treadmill with an increasing load for 11 weeks. Thirty-six hours after the last treadmill training all rats were sacrificed by decapitation to separate their frontal cortex. The method of ELISA was used to detect the content of protein of BDNF, NGF and NT3, real time-PCR was used to determine the mRNA transcription of DBNF, NGF, NT3 and the western blot was used to detect the protein expression of p-CREB. Results： Compared with group C, the levels of BDNF and NGF in group E both increased significantly （ P 〈 0.01 and P 〈 0.05 respectively ） while NT-3 decreased significantly in frontal cortex （ P 〈 0.05 ）. Compared with group E, the levels of BDNF and NGF in group G both decreased significantly in frontal cortex （ P 〈 0. 01 and P 〈 0.05 respectively）. Compared with group C, the expression of BDNF and NGF mRNA in group E were significantly up-regulated in frontal cortex （ P 〈 0.05 for both）. Compared with group E, the expression of BDNF mRNA in group G significantly decreased （P 〈0.05 ）. Compared with group C, the expression of p-CREB in group E increased in frontal cortex; and compared with group E, the expression of p-CREB were down-regulated in frontal cortex in G group. Conclusions： Overtraining can obviously influence the expression of BDNF, NGF, their mRNA and p-CREB in frontal cortex of rat brain. Glutamine supplementation could reverse to a certain degree the expression of BDNF and its mRNA of overtraining rat brain and could also bring about some changes of the level of p-CREB expression.

关 键 词：过度训练 谷氨酰胺 神经营养素 p—CREB 额叶皮质 大鼠 

分 类 号：G804.2[文化科学—运动人体科学]