硒和蛋白质对大鼠心肌蛋白质及DNA修复酶的影响  

The effects of selenium and protein on myocardial protein and DNA repair enzymes of rats

作　　者：王艺如[1,2] 闫丽佳 陈洁 刘明法[5] 李少臣[6] 张辉[7] 刘春旭 裴俊瑞[9] 周令望[10] 侯杰[1] 张立君[1] 王铜[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心克山病研究所,黑龙江哈尔滨150081 [2]天津市红桥区疾病预防控制中心,天津300132 [3]北京市昌平区小汤山社区卫生服务中心,北京100124 [4]北京市朝阳区高碑店社区卫生服务中心保健科,北京102211 [5]天津市塘沽区疾病预防控制中心,天津304500 [6]浙江省嘉兴市秀洲区卫生监督所,浙江嘉兴314000 [7]天津市河北区疾病预防控制中心,天津300150 [8]江苏省南京市浦口区疾病预防控制中心,江苏南京210031 [9]中国疾病预防控制中心地方病控制中心中心实验室,黑龙江哈尔滨150081 [10]中国疾病预防控制中心地方病控制中心大骨节病研究所,黑龙江哈尔滨150081

出　　处：《国外医学（医学地理分册）》2011年第1期18-21,共4页Foreign Medical Sciences：Section of Medgeography

基　　金：国家自然科学基金资助项目(No.30471497);国家自然科学基金资助项目(No.30872191);黑龙江省自然科学基金资助项目(No.ZJY04-07);黑龙江省教育厅科学技术研究项目(No.1151149)

摘　　要：目的研究低硒低蛋白引起的氧化应激对大鼠心肌蛋白质及DNA修复酶的影响。方法 60只健康雄性Wistar大鼠,按体重随机分为4组,每组15只:低硒低蛋白组(A)、常硒低蛋白组(B)、低硒常蛋白组(C)和常硒常蛋白组(D),饲养1年。处死后,采用2,4-二硝基苯肼(DNPH)比色法检测大鼠心肌蛋白质羰基的含量;提取心肌组织总RNA,采用实时荧光定量PCR方法检测DNA修复酶(OGG1和MUTYH)mRNA表达水平;Western blot方法检测DNA修复酶(OGG1和MUTYH)的蛋白表达水平。结果各组之间蛋白质羰基含量的差别没有统计学意义;OGG1和MUTYH的mRNA表达水平,其中均为常硒组的总体均数高于低硒组,常蛋白组的总体均数高于低蛋白组,差异具有统计学意义(P<0.05);MUTYH的蛋白表达,常硒组的总体均数高于低硒组,常蛋白组的总体均数高于低蛋白组,差异具有统计学意义(P<0.05)。结论长期低硒低蛋白饮食可能会影响机体DNA修复酶(OGG1和MUTYH的活力,但是蛋白质羰基的含量没有变化。Objective To investigate the oxidative damage effect of selenium and protein on myocardial protein and DNA repair enzymes in rat myocardium. Methods 6o healthy male Wistar rats were randomly divided into four groups according to their body weight, each group with 15 rats. Group A was low-protein and low- selenium group, group B normal-Se and low-protein group, group C low-selenium and normal-protein group, group D normal-Se and normal-protein group. After being fed for 12 months,all rats were killed and the protein carbonyl in cardiocytes was measured. Total RNA was extracted from heart, and the OGG1 and MUTYH mRNA expressions were detected by real-time PCR. Proteins were extracted from heart and their expressions were detected with Western blot. Results The mRNA expression of OGG1 and MUTYH was higher in the adequate selenium groups than in the selenium deficient groups. The protein expression MUTYH was higher in the adequate selenium groups than in the deficient groups. Conclusion The DNA repair enzymes （OGG1 and MUTYH） may be closely related to selenium.

关 键 词：硒 蛋白质 氧化损伤 蛋白质羰基 DNA修复酶 

分 类 号：R188[医药卫生—流行病学]