白藜芦醇对JurcatT细胞分化为Th1/Th2细胞作用的影响及机制  

Effect and mechanism of resveratrol on the differentiation of Jurcat T cell to Th1/Th2 cell

作　　者：张敏[1] 金呈强[2] 王文涓[1] 刘仿[1] 

机构地区：[1]广东医学院微生物与免疫学教研室,广东湛江524023 [2]济宁医学院附属医院

出　　处：《山东医药》2011年第7期32-34,共3页Shandong Medical Journal

基　　金：广东省自然科学基金资助项目(06028959);广东医学院青年基金资助项目(XQ06017);广东省湛江市科技计划项目(2009C3103019)

摘　　要：目的探讨白藜芦醇调节Jurkat T细胞分泌为Th1/Th2型细胞作用的影响。方法将不同浓度的白藜芦醇作用于受植物血凝素(PHA)刺激的Jurkat T细胞,于不同时间点分别用ELISA法检测Th1型细胞因子IFN-γ和Th2型细胞因子IL-10;RT-PCR法检测T-bet和GATA-3 mRNA表达。结果白藜芦醇作用后IFN-γ、IL-10及T-bet mRNA、GATA-3 mRNA的表达均明显降低,具有浓度和时间依赖性。结论白藜芦醇对受PHA刺激的JurcatT细胞由Th0向Th1、Th2细胞分化起抑制作用。ObjectiveTo approach the effect of resveratrol on the differentiation of Jurcat T cell to Th1/Th2 cell.MethodsJurkat T cells stimulated by phytohemagglutinin（PHA） were treated with different concentrations of resveratrol,and Th1 cell related cytokine IFN-γ and Th2 cell related cytokine IL-10 were detected by ELISA,the expression of T-bet and GATA-3 mRNA were detected by RT-PCR.ResultsThe level of IFN-γ,IL-10 and expression of T-bet and GATA-3 mRNA all decreased significantly,and showed dose and time-dependent manner.ConclusionsResveratrol can inhibit the differentiation of Jurcat T cell to Th1/Th2 cell.

关 键 词：白藜芦醇 TH1 TH2 分化作用 

分 类 号：R714.246[医药卫生—妇产科学]