反义血管紧张素Ⅱ受体Ⅰ_B基因转移对大鼠颈动脉损伤后新生内膜的影响  

Effects of adenoviral vector-mediated rat antisense AT_(1B) gene transfer on neointima proliferation after rat carotid artery injury

作　　者：欧阳平[1] 许顶立[1] 黄洪莲 刘伊丽[1] 侯玉清[1] 宋后燕[3] 戴云 

机构地区：[1]第一军医大学南方医院心内科,广州510515 [2]第一军医大学基础部 [3]上海医科大学分子遗传研究室

出　　处：《中华心血管病杂志》1999年第5期381-383,共3页Chinese Journal of Cardiology

摘　　要：目的　探讨腺病毒载体介导的大鼠反义血管紧张素Ⅱ受体ⅠB(AT1B)RNA转移对大鼠颈动脉损伤后新生内膜的影响。方法　利用重组腺病毒载体将大鼠反义AT1B RNA转移至大鼠颈动脉球囊损伤模型中 ,2 1天后观察其对新生内膜形成的影响。结果　用腺病毒载体转导大鼠反义AT1BRNA至大鼠球囊损伤的颈动脉 ,与对照组相比 ,2 1天后损伤血管新生内膜 /中层面积比降低了 4 7%(P <0 0 0 1)。结论　反义AT1BRNA对血管成形术后再狭窄可能有一定的预防作用。Objective To study the effects of rat angiotensin II receptor I B (antisense AT 1B ) gene transfer mediated by adenoviral vector on neointimal proliferation after rat carotid injury.Methods Antisense (AT 1B ) gene was transducted into the carotid artery by adenoviral vector after carotid balloon injury and the restenosis model was established in SD rat. Neointima/media area ratio in local artery at day 21 after gene transfer was measured.Results Rat antisense AT 1B gene was succesfully transducted into the carotid artery after carotid balloon injury. Neointima/media area ratio was significantly reduced (47%, P <0.01) compared with the control group at day 21 after gene transfer.Conclusion The results suggest the possibility that antisense AT 1B gene transfer is a potential therapeutic approach to prevent neointimal hyperplasia.

关 键 词：颈动脉损伤 血管内膜 基因治疗 AT1B 

分 类 号：R543.405[医药卫生—心血管疾病]