暗紫贝母有效成分提取工艺研究  

Study on the Extraction Process of Active Ingredients from F.unibracteata Hsiao et K.C.Hsia

作　　者：韩鸿萍[1] 杜晓[2] 

机构地区：[1]青海师范大学化学系,青海西宁810008 [2]青海师范大学生命与地理科学学院,青海西宁810008

出　　处：《安徽农业科学》2011年第6期3288-3290,共3页Journal of Anhui Agricultural Sciences

基　　金：科技部科技支撑项目(2007BAI45B04);青海省科技厅项目(2007-T-03)

摘　　要：[目的]优化暗紫贝母(Fritillaria unibracteata Hsiao et K.C.Hsia)中总生物碱的提取工艺。[方法]在单因素试验基础上,采用正交试验L36(42×32)对暗紫贝母中已知的活性成分总生物碱的提取工艺进行优化。[结果]暗紫贝母中有效成分的最佳提取工艺为:样品粉末粒径200目,乙醇体积分数75%,提取时间90 min,提取1次。其中,提取次数对提取结果影响极显著(P<0.01);样品粉末粒径对提取效果的影响显著(P<0.05);提取时间和乙醇体积分数对提取效果的影响不显著。[结论]该研究可为暗紫贝母的制剂生产、质量控制、合理利用及开发贝母属植物资源提供科学依据。[Object] To optimize the extraction process of total alkaloids from F.unibracteata Hsiao et K.C.Hsia.[Method] Based on the single factor test,the extraction process of total alkaloids from F.unibracteata Hsiao et K.C.Hsia has been optimized through orthogonal test L36（42×32）.[Result] The optimum extraction process was as follows： partical size of 200 mesh,alcohol fraction of 75%,extraction duration of 30 min,extraction time of one,among which extraction time had extremely significant effect on the result（P0.01﹚,the partical size had significant effect on the extraction result（P0.05﹚,extraction duration and alcohol fraction had no significant influence on the result.[Conclusion] The study would provide scientific references for the production,quality control and reasonable utilization of F.unibracteata Hsiao et K.C.Hsia resources.

关 键 词：暗紫贝母 正交设计 酸性染料比色法 总生物碱 提取工艺 

分 类 号：S567.251[农业科学—中草药栽培]