女贞子总黄酮提取工艺的研究  

Study on the Extraction of Total Flavonoids from FRUCTUS LIGUSTRI LUCIDI

作　　者：徐玲花[1] 熊亚青[1] 严镇钧[2] 杨裕启[1] 吴雪丽[1] 吴习姣[1] 

机构地区：[1]黄石理工学院化学与材料工程学院,湖北黄石435003 [2]湖北师范学院生命科学学院,湖北黄石435002

出　　处：《安徽农业科学》2011年第6期3305-3308,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优化女贞子(FRUCTUS LIGUSTRI LUCIDI)中总黄酮的提取工艺。[方法]在单因素试验基础上,采用L9(34)正交试验对乙醇回流法提取女贞子总黄酮的工艺进行优化。[结果]最佳提取工艺条件为:2.50 g干燥女贞子粉末,乙醇体积分数60%,料液比1∶50(M/V),提取温度85℃,提取时间2 h。在此最佳提取工艺条件下,总黄酮提取率较高,为6.215%。[结论]乙醇回流法提取女贞子总黄酮稳定可靠。[Objective] To optimize the extraction process of total flavonoids from FRUCTUS LIGUSTRI LUCIDI.[Method] On the basis of single test,the extraction process was optimized through L9（34） orthogonal test.[Result] The optimum extraction process was as follows：2.50 g dried powder of FRUCTUS LIGUSTRI LUCIDI,60% alcohol as extractant,solid-liquid of 85 ℃,extraction duration of 2 h,under the above condition the yield rate of total flavonoids reached as high as 6.215%.[Conclusion] Ethanol reflux method was proved to be stable and reliable for extracting total flavonoids from FRUCTUS LIGUSTRI LUCIDI.

关 键 词：女贞子 黄酮 提取工艺 

分 类 号：S567.239[农业科学—中草药栽培]