夹竹桃叶色白化的原因探讨  

Research on the Reason of Albino Oleander Leaf

作　　者：王永亮[1] 赵歌[1] 李叶峰[1] 杨燕[1] 陆小平[1] 

机构地区：[1]苏州大学金螳螂建筑与城市环境学院,江苏苏州215123

出　　处：《安徽农业科学》2011年第6期3466-3467,3472,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]从叶片结构,叶绿体等亚细胞结构以及色素组成研究该突变体叶色白化的原因。[方法]以叶色白化的夹竹桃叶片为材料,用光学显微镜观察不同发育阶段的叶绿体形态;用板层析技术分析叶片中光合色素的组成。[结果]突变型与野生型的叶肉细胞无明显差异,但白化叶片中栅栏组织和海绵组织几乎看不到绿色存在;分离2种叶色的叶绿体发现,突变型的成熟叶绿体数量很少;板层析结果显示,白化夹竹桃中光合色素基本缺失。[结论]夹竹桃叶色白化可能是因叶绿体合成受阻引起叶绿素缺失造成的。[Objective] The reason of the albino mutant of oleander leaf was researched from the structure of leaf,the chloroplast in leaf,the structure of sub-cellular and the pigment composition of leaf.[Method] The chloroplast form of albino leaf of oleander in different developmental stages was observed under optical microscope and the composition of the photosynthetic pigment in albino leaf was analyzed with the technique of plate chromatography.[Results] There was no significant difference in mesophyll cell between the mutant and wild species of oleander,however in the palisade and spongy tissue of albino leaf the green could barely be seen.The experiment in the isolation of chloroplast from the normal plant and mutant indicated that few mature chloroplasts could be found in the mutant.The result from the analysis of plate chromatography showed that there was basically no photosynthetic pigment in the albino leaf.[Conclusion] The reason causing the albino leaf of oleander might be the lack of the chlorophyll due to the disruption of the chloroplast synthesis in leaf.

关 键 词：白叶夹竹桃 叶绿体 光合色素 

分 类 号：D685.99[政治法律—政治学]