甘蔗汁对大鼠酒精性肝损伤的保护作用  

Research of the Hepatoprotective Effects of Sugar Cane Juice against Alcohol-induced Liver Injury in Rats

作　　者：韦日明[1] 王凌宇[1] 肖胜军[2] 彭新青[3] 肖福英[1] 夏春波[4] 吴群英[1] 蒋林彬[1] 刘健翔[3] 

机构地区：[1]桂林医学院细胞与遗传学教研室,广西桂林541004 [2]桂林医学院病理学教研室,广西桂林541004 [3]桂林医学院生物技术教研室,广西桂林541004 [4]桂林医学院解剖学教研室,广西桂林541004

出　　处：《安徽农业科学》2011年第6期3606-3606,3661,共2页Journal of Anhui Agricultural Sciences

基　　金：广西教育厅科研项目[(桂教科研34号)200710LX354]

摘　　要：[目的]通过建立大鼠酒精性肝损伤病理模型,探讨甘蔗汁对大鼠酒精性肝损伤的保护作用。[方法]取大鼠40只随机分为4组,正常组、低剂量组、高剂组量和模型组。采用白酒灌胃的方法建立大鼠酒精性肝病模式,给予甘蔗汁进行保护。测定肝组织中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量,并取肝组织制作石蜡切片,HE染色,普通光镜观察。[结果]甘蔗汁能够直接提高SOD活性、降低MDA含量,且低剂量(7.5 m l/kg)和高剂量(15 m l/kg)能够有效清除活性氧和自由基,明显改善肝组织脂肪样变。[结论]甘蔗汁能抑制酒精诱导脂质过氧化反应对肝组织的损伤,对酒精性肝损伤有明显的保护作用。[Objective] The aim was to investigate the hepatoprotective effects of sugar cane juice through establishing alcohol-induced liver injury pathology model of rats.[Method] Forty rats were randomly divided into 4 groups,including natural group,low-dosage group,high-dosage group and model group.Duplicated the animal models of alcohol-induced liver injury by the way of gaster ＂san hua＂ alcohol given,then gave the sugar cane juice to carry on the protection.Superoxide dismutase（SOD） activity,malonyldiadehyde（MDA）content in liver tissue were determined respectively in all experimental rats.Liver tissue preparations were stained with HE staining.[Result] Sugar cane juice can directly increase the activity of SOD,and decrease the content of MDA in liver tissue.Both low dose（7.5 ml/kg）and high dose（15 ml/kg） can effectively clear up reactive oxygen species（ROS） and free radical,and improve the liver histology lipoid degeneration.[Conclusion] Sugar cane juice can effectively inhibit the lipid-peroxidation-infuced injury in liver,showed obvious protective effects against alcohol-induced liver injury.

关 键 词：甘蔗汁 酒精 肝损伤 保护作用 

分 类 号：S566.1[农业科学—作物学]