四羟甲基硫酸鏻对日本鳗鲡的急性毒性及杀菌性能试验  

Acute Toxicity Test of Japanese eel and Bactericidal Property Test of Tetrakis Hydroxymethyl Phosphonium Sulfate

作　　者：林明辉[1] 刘春花[1] 陈道印 黄志斌[1] 孙保兴 

机构地区：[1]中国水产科学研究院珠江水产研究所,广东广州510280 [2]南昌县莲塘鱼病防治所,江西南昌330200 [3]广州市畅洋绿色动物药品有限公司,广东广州511450

出　　处：《安徽农业科学》2011年第6期3647-3648,3689,共3页Journal of Anhui Agricultural Sciences

基　　金：农业部公益性行业科研专项(200803013);珠江水产研究所所长基金(2007-G-6)

摘　　要：[目的]探讨四羟甲基硫酸鏻(THPS)作为一种新型季鏻盐杀生剂对日本鳗鲡的毒性作用以及杀菌效果。[方法]在室内常温静水条件下,进行THPS对日本鳗鲡(Anguilla japonica)急性毒性试验,以及THPS对柱形屈绕杆菌(F.columnaris)的杀菌性能试验。计算THPS的半致死浓度(LC50)、24 h安全浓度(Sc)、对柱形屈绕杆菌的最低抑菌浓度(MIC)、最低杀菌浓度(MBC)以及杀菌率。[结果]THPS的24、48和96 h的LC50分别为11.9、9.9和9.1 mg/L;Sc为2.1 mg/L;MIC和MBC分别为0.36和0.72 mg/L;浓度为0.7 mg/L,作用12 h的杀菌率达100.0%,药效持久,且受pH值影响极小。[结论]新型季鏻盐杀生剂对防治鳗鲡细菌性疾病安全、有效,值得推广。[Objective] To understand the toxicity and sterilizing effects of the tetrakis hydroxmethyl phosphonium sulfate（THPS） as a novel quaternary phosphonium biocide.[Method] Under hydrostatic conditions at RT,the acute toxicity test of Japanese eel and the bactericidal property test of THPS on the F.columnaris were carried out.And then the semi-lethal concentration（LC50）,safe concentration（Sc）,minimum inhibitory concentration（MIC）,minimum bactericidal concentration（MBC） and the sterilizing rate of THPS were calculated,respectively.[Result] The LC50of THPS at 24,48 and 96 h were 11.9,9.9 and 9.1 mg/L,respectively.The Sc of THPS at 24 h was 2.1 mg/L;the MIC and MBC of THPS to the F.columnaris were 0.36 and 0.72 mg/L;and the sterilizing rate was 100.0% at a concentration of 0.7 mg/L and 12 h.The sterilizing rate of THPS had no great change with pH values varying from 5.5 to 9.5.[Conclusion] The THPS is a novel,safe and effective biocide for prevention and treatment of bacterial diseases of Japanese eel.

关 键 词：四羟甲基硫酸鏻 日本鳗鲡 柱形屈绕杆菌 急性毒性 杀菌性能 

分 类 号：S482.2[农业科学—农药学]